A Novel Retron of Vibrio Parahaemolyticus is Closely Related to Retron-Vc95 of Vibrio Cholerae

Overview

Journal J Microbiol

Specialty Microbiology

Date 2013 Jul 2

PMID 23812812

Citations 1

Authors

Toshi Shimamoto

Ashraf M Ahmed

Tadashi Shimamoto

Affiliations

Soon will be listed here.

Abstract

Some bacteria produce a satellite RNA-DNA complex termed msDNA, multicopy single-stranded DNA. In this report, msDNA from Vibrio parahaemolyticus, a cause of acute gastroenteritis, was identified and named msDNA-Vp96. The retron element containing the ret gene, encoding the reverse transcriptase (RT) that is responsible for msDNA production, was cloned and characterized. Comparison of msDNA-Vp96 and msDNA-Vc95, from Vibrio cholerae, showed a high level of sequence similarity. We exchanged the two ret genes to examine whether msDNA was produced by the RT from different sources. We found that RT-Vp96 of V. parahaemolyticus was able to synthesize msDNA-Vc95 of V. cholerae and vice versa. To the best of our knowledge, this is the first report that RT from different bacterial species can synthesize msDNA.

Citing Articles

Retrons and their applications in genome engineering.

Simon A, Ellington A, Finkelstein I Nucleic Acids Res. 2019; 47(21):11007-11019.

PMID: 31598685 PMC: 6868368. DOI: 10.1093/nar/gkz865.

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