Molecular Heterogeneity of Large-conductance Calcium-activated Potassium Channels in Canine Intracardiac Ganglia

Overview

Journal Channels (Austin)

Specialty Biochemistry

Date 2013 Jun 29

PMID 23807090

Citations 4

Authors

Elisabet Selga

Alexandra Perez-Serra

Alba Moreno-Asso

Seth Anderson

Kristen Thomas

Mayurika Desai

Ramon Brugada

Guillermo J Perez

Fabiana S Scornik

Affiliations

Soon will be listed here.

Abstract

Large conductance calcium-activated potassium (BK) channels are widely expressed in the nervous system. We have recently shown that principal neurons from canine intracardiac ganglia (ICG) express a paxilline- and TEA-sensitive BK current, which increases neuronal excitability. In the present work, we further explore the molecular constituents of the BK current in canine ICG. We found that the β1 and β4 regulatory subunits are expressed in ICG. Single channel voltage-dependence at different calcium concentrations suggested that association of the BKα with a particular β subunit was not enough to explain the channel activity in this tissue. Indeed, we detected the presence of several splice variants of the BKα subunit. In conclusion, BK channels in canine ICG may result from the arrangement of different BKα splice variants, plus accessory β subunits. The particular combinations expressed in canine IC neurons likely rule the excitatory role of BK current in this tissue.

Citing Articles

Conserved Role of the Large Conductance Calcium-Activated Potassium Channel, K1.1, in Sinus Node Function and Arrhythmia Risk.

Pineda S, Nikolova-Krstevski V, Leimena C, Atkinson A, Altekoester A, Cox C Circ Genom Precis Med. 2021; 14(2):e003144.

PMID: 33629867 PMC: 8058291. DOI: 10.1161/CIRCGEN.120.003144.

Elucidating the Role of K Channels during In Vitro Capacitation of Boar Spermatozoa: Do SLO1 Channels Play a Crucial Role?.

Yeste M, Llavanera M, Perez G, Scornik F, Puig-Parri J, Brugada R Int J Mol Sci. 2019; 20(24).

PMID: 31847486 PMC: 6940911. DOI: 10.3390/ijms20246330.

Synthesis and Biological Evaluation of New Tricyclic Dihydropyridine Based Derivatives on Potassium Channels.

Gunduz M, Kaya Y, Simsek R, Sahin-Erdemli I, Safak C Iran J Pharm Res. 2017; 15(4):763-775.

PMID: 28243272 PMC: 5316254.

The smooth muscle-type β1 subunit potentiates activation by DiBAC4(3) in recombinant BK channels.

Bosch Calero C, Selga E, Brugada R, Scornik F, Perez G Channels (Austin). 2013; 8(1):95-102.

PMID: 24299688 PMC: 4048348. DOI: 10.4161/chan.27212.

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