Use of a Synthetic Oligonucleotide Probe to Detect Strains of Non-serovar O1 Vibrio Cholerae Carrying the Gene for Heat-stable Enterotoxin (NAG-ST)

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1990 Jun 1

PMID 2380369

Citations 12

Authors

C W Hoge

O Sethabutr

L Bodhidatta

P Echeverria

D C ROBERTSON

J G Morris Jr

Affiliations

Soon will be listed here.

Abstract

A synthetic oligonucleotide probe was developed to identify the gene for the heat-stable enterotoxin (NAG-ST) of non-serovar O1 Vibrio cholerae. Of 103 non-O1 V. cholerae isolates from Thailand, 31 isolates from Mexico, and 47 isolates from patients in the United States, only 7 (all from Thailand) hybridized with the probe. Probe-positive strains produced significantly higher fluid accumulations in infant mice than probe-negative strains.

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