Intracellular Fate of Carbon Nanotubes Inside Murine Macrophages: PH-dependent Detachment of Iron Catalyst Nanoparticles

Overview

Journal Part Fibre Toxicol

Publisher Biomed Central

Specialty Toxicology

Date 2013 Jun 27

PMID 23800198

Citations 10

Authors

Cyrill Bussy

Erwan Paineau

Julien Cambedouzou

Nathalie Brun

Claudie Mory

Barbara Fayard

Murielle Salome

Mathieu Pinault

Mickael Huard

Esther Belade

Lucie Armand

Jorge Boczkowski

Pascale Launois

Sophie Lanone

Affiliations

Soon will be listed here.

Abstract

Background: Carbon nanotubes (CNT) are a family of materials featuring a large range of length, diameter, numbers of walls and, quite often metallic impurities coming from the catalyst used for their synthesis. They exhibit unique physical properties, which have already led to an extensive development of CNT for numerous applications. Because of this development and the resulting potential increase of human exposure, an important body of literature has been published with the aim to evaluate the health impact of CNT. However, despite evidences of uptake and long-term persistence of CNT within macrophages and the central role of those cells in the CNT-induced pulmonary inflammatory response, a limited amount of data is available so far on the CNT fate inside macrophages. Therefore, the overall aim of our study was to investigate the fate of pristine single walled CNT (SWCNT) after their internalization by macrophages.

Methods: To achieve our aim, we used a broad range of techniques that aimed at getting a comprehensive characterization of the SWCNT and their catalyst residues before and after exposure of murine macrophages: X-ray diffraction (XRD), High Resolution (HR) Transmission Electron Microscopy (TEM), High Angle Annular Dark Field-Scanning TEM (HAADF-STEM) coupled to Electron Energy Loss Spectroscopy (EELS), as well as micro-X-ray fluorescence mapping (μXRF), using synchrotron radiation.

Results: We showed 1) the rapid detachment of part of the iron nanoparticles initially attached to SWCNT which appeared as free iron nanoparticles in the cytoplasm and nucleus of CNT-exposed murine macrophages, and 2) that blockade of intracellular lysosomal acidification prevented iron nanoparticles detachment from CNT bundles and protected cells from CNT downstream toxicity.

Conclusions: The present results, while obtained with pristine SWCNT, could likely be extended to other catalyst-containing nanomaterials and surely open new ways in the interpretation and understanding of CNT toxicity.

Citing Articles

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Mitochondrial Impairment Induced by Sub-Chronic Exposure to Multi-Walled Carbon Nanotubes.

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Carbon nanotubes, but not spherical nanoparticles, block autophagy by a shape-related targeting of lysosomes in murine macrophages.

Cohignac V, Landry M, Ridoux A, Pinault M, Annangi B, Gerdil A Autophagy. 2018; 14(8):1323-1334.

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Cammisuli F, Giordani S, Gianoncelli A, Rizzardi C, Radillo L, Zweyer M Sci Rep. 2018; 8(1):706.

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