Evaluating Stem and Cancerous Biomarkers in CD15+CD44+ KYSE30 Cells

Overview

Journal Tumour Biol

Publisher Sage Publications

Specialty Oncology

Date 2013 Jun 26

PMID 23797812

Citations 9

Authors

Fatemeh B Rassouli

Maryam M Matin

Ahamd Reza Bahrami

Kamran Ghaffarzadegan

Hamid Cheshomi

Sara Lari

Bahram Memar

Mun Seng Kan

Affiliations

Soon will be listed here.

Abstract

Digestive system cancers are listed among the ten top causes of cancer-related death worldwide. Cancer stem cells (CSCs) are malignant cells that share some of their characteristics with normal stem cells, including self-renewal and multipotency, and also cancer cells, such as drug resistance and metastasis. Despite many reports on CSCs with digestive system origin, identification and characterization of esophageal CSCs have remained elusive. To examine the validity of routine SC, cancer cell and CSC markers in KYSE30 cells, derived from esophageal carcinoma, cells were first characterized by immunofluorescence and RT-PCR techniques, and then the significance of candidate biomarkers was evaluated in retinoic acid-treated cells by flow cytometry and/or real-time RT-PCR. Meanwhile, to study CD15 (a newly introduced CSC marker) expression in digestive tract cancers, human normal and tumoral tissues of esophagus, stomach, and colon were analyzed by immunohistochemistry. Using several experimental approaches, we show that CD44, but not CD15, could serve as a reliable marker for undifferentiated malignant squamous cells of esophagus. In conclusion, our study confirms the role of CD44 as a CSC marker in KYSE30 cells, an esophageal squamous cell carcinoma cell line, and for the first time indicates the expression of CD15 in non-neural stem-like cancer cells. Although the importance of CD15 was not indicated in diagnosis of digestive cancers, further studies are needed to better understand the biological identity and function of this molecule in non-neural malignancies.

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References

Wanner R, Panteleyev A, Henz B, Rosenbach T . Retinoic acid affects the expression rate of the differentiation-related genes aryl hydrocarbon receptor, ARNT and keratin 4 in proliferative keratinocytes only. Biochim Biophys Acta. 1996; 1317(2):105-11. DOI: 10.1016/s0925-4439(96)00038-5. View

Saiki Y, Ishimaru S, Mimori K, Takatsuno Y, Nagahara M, Ishii H . Comprehensive analysis of the clinical significance of inducing pluripotent stemness-related gene expression in colorectal cancer cells. Ann Surg Oncol. 2009; 16(9):2638-44. DOI: 10.1245/s10434-009-0567-5. View

Pentenero M, Gandolfo S, Carrozzo M . Importance of tumor thickness and depth of invasion in nodal involvement and prognosis of oral squamous cell carcinoma: a review of the literature. Head Neck. 2005; 27(12):1080-91. DOI: 10.1002/hed.20275. View

Strong K, Mathers C, Epping-Jordan J, Resnikoff S, Ullrich A . Preventing cancer through tobacco and infection control: how many lives can we save in the next 10 years?. Eur J Cancer Prev. 2008; 17(2):153-61. DOI: 10.1097/CEJ.0b013e3282b6fda8. View

Bapat S . Human ovarian cancer stem cells. Reproduction. 2010; 140(1):33-41. DOI: 10.1530/REP-09-0389. View

Andrews P, Nudelman E, Hakomori S, Fenderson B . Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoic acid, hexamethylene bisacetamide (HMBA) or bromodeoxyuridine (BUdR). Differentiation. 1990; 43(2):131-8. DOI: 10.1111/j.1432-0436.1990.tb00439.x. View

Strakova Z, Livak M, Krezalek M, Ihnatovych I . Multipotent properties of myofibroblast cells derived from human placenta. Cell Tissue Res. 2008; 332(3):479-88. PMC: 2536756. DOI: 10.1007/s00441-008-0604-x. View

Naor D, Wallach-Dayan S, Zahalka M, Sionov R . Involvement of CD44, a molecule with a thousand faces, in cancer dissemination. Semin Cancer Biol. 2008; 18(4):260-7. DOI: 10.1016/j.semcancer.2008.03.015. View

Li C, Heidt D, Dalerba P, Burant C, Zhang L, Adsay V . Identification of pancreatic cancer stem cells. Cancer Res. 2007; 67(3):1030-7. DOI: 10.1158/0008-5472.CAN-06-2030. View

10.

Takaishi S, Okumura T, Tu S, Wang S, Shibata W, Vigneshwaran R . Identification of gastric cancer stem cells using the cell surface marker CD44. Stem Cells. 2009; 27(5):1006-20. PMC: 2746367. DOI: 10.1002/stem.30. View

11.

Virtanen M, Sirsjo A, Vahlquist A, Torma H . Keratins 2 and 4/13 in reconstituted human skin are reciprocally regulated by retinoids binding to nuclear receptor RARalpha. Exp Dermatol. 2010; 19(7):674-81. DOI: 10.1111/j.1600-0625.2010.01079.x. View

12.

Uchida N, Buck D, He D, Reitsma M, Masek M, Phan T . Direct isolation of human central nervous system stem cells. Proc Natl Acad Sci U S A. 2000; 97(26):14720-5. PMC: 18985. DOI: 10.1073/pnas.97.26.14720. View

13.

Saigusa S, Tanaka K, Toiyama Y, Yokoe T, Okugawa Y, Ioue Y . Correlation of CD133, OCT4, and SOX2 in rectal cancer and their association with distant recurrence after chemoradiotherapy. Ann Surg Oncol. 2009; 16(12):3488-98. DOI: 10.1245/s10434-009-0617-z. View

14.

Huang D, Gao Q, Guo L, Zhang C, Jiang W, Li H . Isolation and identification of cancer stem-like cells in esophageal carcinoma cell lines. Stem Cells Dev. 2008; 18(3):465-73. DOI: 10.1089/scd.2008.0033. View

15.

Long K, Hornick J . SOX2 is highly expressed in squamous cell carcinomas of the gastrointestinal tract. Hum Pathol. 2009; 40(12):1768-73. DOI: 10.1016/j.humpath.2009.06.006. View

16.

Dean M, Fojo T, Bates S . Tumour stem cells and drug resistance. Nat Rev Cancer. 2005; 5(4):275-84. DOI: 10.1038/nrc1590. View

17.

Sanada Y, Yoshida K, Ohara M, Oeda M, Konishi K, Tsutani Y . Histopathologic evaluation of stepwise progression of pancreatic carcinoma with immunohistochemical analysis of gastric epithelial transcription factor SOX2: comparison of expression patterns between invasive components and cancerous or.... Pancreas. 2006; 32(2):164-70. DOI: 10.1097/01.mpa.0000202947.80117.a0. View

18.

Al-Hajj M, Wicha M, Benito-Hernandez A, Morrison S, Clarke M . Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci U S A. 2003; 100(7):3983-8. PMC: 153034. DOI: 10.1073/pnas.0530291100. View

19.

Virtanen M, Torma H, Vahlquist A . Keratin 4 upregulation by retinoic acid in vivo: a sensitive marker for retinoid bioactivity in human epidermis. J Invest Dermatol. 2000; 114(3):487-93. DOI: 10.1046/j.1523-1747.2000.00901.x. View

20.

Baccelli I, Trumpp A . The evolving concept of cancer and metastasis stem cells. J Cell Biol. 2012; 198(3):281-93. PMC: 3413352. DOI: 10.1083/jcb.201202014. View