A Liposome-based Ion Release Impedance Sensor for Biological Detection

Overview

Journal Biomed Microdevices

Specialty Biomedical Engineering

Date 2013 Jun 25

PMID 23793417

Citations 3

Authors

Gregory L Damhorst

Cartney E Smith

Eric M Salm

Magdalena M Sobieraj

Hengkan Ni

Hyunjoon Kong

Rashid Bashir

Affiliations

Soon will be listed here.

Abstract

Low-cost detection of pathogens and biomolecules at the point-of-care promises to revolutionize medicine through more individualized monitoring and increased accessibility to diagnostics in remote and resource-limited areas. While many approaches to biosensing are still limited by expensive components or inadequate portability, we present here an ELISA-inspired lab-on-a-chip strategy for biological detection based on liposome tagging and ion-release impedance spectroscopy. Ion-encapsulating dipalmitoylphosphatidylcholine (DPPC) liposomes can be functionalized with antibodies and are stable in deionized water yet permeabilized for ion release upon heating, making them ideal reporters for electrical biosensing of surface-immobilized antigens. We demonstrate the quantification of these liposomes by real-time impedance measurements, as well as the qualitative detection of viruses as a proof-of-concept toward a portable platform for viral load determination which can be applied broadly to the detection of pathogens and other biomolecules.

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