The Effect of RhBMP-2 and PRP Delivery by Biodegradable β-tricalcium Phosphate Scaffolds on New Bone Formation in a Non-through Rabbit Cranial Defect Model

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2013 Jun 20

PMID 23779152

Citations 12

Authors

Hyun-Pil Lim

Angel E Mercado-Pagan

Kwi-Dug Yun

Seong-Soo Kang

Taek-Hue Choi

Julius Bishop

Jeong-Tae Koh

William Maloney

Kwang-Min Lee

Yunzhi Peter Yang

Sang-Won Park

Affiliations

Soon will be listed here.

Abstract

This study evaluated whether the combination of biodegradable β-tricalcium phosphate (β-TCP) scaffolds with recombinant human bone morphogenetic protein-2 (rhBMP-2) or platelet-rich plasma (PRP) could accelerate bone formation and increase bone height using a rabbit non-through cranial bone defect model. Four non-through cylindrical bone defects with a diameter of 8-mm were surgically created on the cranium of rabbits. β-TCP scaffolds in the presence and absence of impregnated rhBMP-2 or PRP were placed into the defects. At 8 and 16 weeks after implantation, samples were dissected and fixed for analysis by microcomputed tomography and histology. Only defects with rhBMP-2 impregnated β-TCP scaffolds showed significantly enhanced bone formation compared to non-impregnated β-TCP scaffolds (P < 0.05). Although new bone was higher than adjacent bone at 8 weeks after implantation, vertical bone augmentation was not observed at 16 weeks after implantation, probably due to scaffold resorption occurring concurrently with new bone formation.

Citing Articles

Chitosan/Sodium Tripolyphosphate/Sodium Alginate/miRNA-34c-5p Antagomir Scaffolds Promote the Functionality of Rabbit Cranial Parietal Repair.

Lin C, Bai X, Zhang L Int J Nanomedicine. 2024; 19:12939-12956.

PMID: 39651352 PMC: 11622683. DOI: 10.2147/IJN.S481965.

Synthetic materials in craniofacial regenerative medicine: A comprehensive overview.

Yazdanian M, Alam M, Abbasi K, Rahbar M, Farjood A, Tahmasebi E Front Bioeng Biotechnol. 2022; 10:987195.

PMID: 36440445 PMC: 9681815. DOI: 10.3389/fbioe.2022.987195.

A Biomimetic Platelet-Rich Plasma-Based Interpenetrating Network Printable Hydrogel for Bone Regeneration.

Tang S, Wang L, Zhang Y, Zhang F Front Bioeng Biotechnol. 2022; 10:887454.

PMID: 35497349 PMC: 9041706. DOI: 10.3389/fbioe.2022.887454.

Clinical Effectiveness of Platelet-Rich Plasma for Long-Bone Delayed Union and Nonunion: A Systematic Review and Meta-Analysis.

Li S, Xing F, Luo R, Liu M Front Med (Lausanne). 2022; 8:771252.

PMID: 35145974 PMC: 8822232. DOI: 10.3389/fmed.2021.771252.

Extraction socket grafting using recombinant human bone morphogenetic protein-2-clinical implications and histological observations.

Durge K, Baliga V, Sridhar S, Dhadse P, Ragit G BMC Res Notes. 2021; 14(1):61.

PMID: 33622406 PMC: 7903772. DOI: 10.1186/s13104-021-05476-0.

References

Chow L . Calcium phosphate cements. Monogr Oral Sci. 2002; 18:148-63. DOI: 10.1159/000061653. View

Marx R, Carlson E, Eichstaedt R, Schimmele S, Strauss J, Georgeff K . Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998; 85(6):638-46. DOI: 10.1016/s1079-2104(98)90029-4. View

Xu X, Lou J, Tang T, Ng K, Zhang J, Yu C . Evaluation of different scaffolds for BMP-2 genetic orthopedic tissue engineering. J Biomed Mater Res B Appl Biomater. 2005; 75(2):289-303. DOI: 10.1002/jbm.b.30299. View

Cabbar F, Guler N, Kurkcu M, Iseri U, Sencift K . The effect of bovine bone graft with or without platelet-rich plasma on maxillary sinus floor augmentation. J Oral Maxillofac Surg. 2011; 69(10):2537-47. DOI: 10.1016/j.joms.2011.03.040. View

Gumieiro E, Abrahao M, Jahn R, Segretto H, Alves M, Nannmark U . Platelet-rich plasma in bone repair of irradiated tibiae of Wistar rats. Acta Cir Bras. 2010; 25(3):257-63. DOI: 10.1590/s0102-86502010000300007. View

Wada T, Hara K, Ozawa H . Ultrastructural and histochemical study of beta-tricalcium phosphate resorbing cells in periodontium of dogs. J Periodontal Res. 1989; 24(6):391-401. DOI: 10.1111/j.1600-0765.1989.tb00888.x. View

Sanchez A, Sheridan P, Kupp L . Is platelet-rich plasma the perfect enhancement factor? A current review. Int J Oral Maxillofac Implants. 2003; 18(1):93-103. View

Kang Y, Scully A, Young D, Kim S, Tsao H, Sen M . Enhanced mechanical performance and biological evaluation of a PLGA coated β-TCP composite scaffold for load-bearing applications. Eur Polym J. 2011; 47(8):1569-1577. PMC: 3164545. DOI: 10.1016/j.eurpolymj.2011.05.004. View

Tazaki J, Murata M, Akazawa T, Yamamoto M, Ito K, Arisue M . BMP-2 release and dose-response studies in hydroxyapatite and beta-tricalcium phosphate. Biomed Mater Eng. 2009; 19(2-3):141-6. DOI: 10.3233/BME-2009-0573. View

10.

Urist M . Bone: formation by autoinduction. Science. 1965; 150(3698):893-9. DOI: 10.1126/science.150.3698.893. View

11.

Levengood S, Polak S, Poellmann M, Hoelzle D, Maki A, Clark S . The effect of BMP-2 on micro- and macroscale osteointegration of biphasic calcium phosphate scaffolds with multiscale porosity. Acta Biomater. 2010; 6(8):3283-91. DOI: 10.1016/j.actbio.2010.02.026. View

12.

Anitua E . The use of plasma-rich growth factors (PRGF) in oral surgery. Pract Proced Aesthet Dent. 2001; 13(6):487-93; quiz 487-93. View

13.

Karande T, Ong J, Agrawal C . Diffusion in musculoskeletal tissue engineering scaffolds: design issues related to porosity, permeability, architecture, and nutrient mixing. Ann Biomed Eng. 2005; 32(12):1728-43. DOI: 10.1007/s10439-004-7825-2. View

14.

Perri B, Cooper M, Lauryssen C, Anand N . Adverse swelling associated with use of rh-BMP-2 in anterior cervical discectomy and fusion: a case study. Spine J. 2007; 7(2):235-9. DOI: 10.1016/j.spinee.2006.04.010. View

15.

Forslund C . BMP treatment for improving tendon repair. Studies on rat and rabbit Achilles tendons. Acta Orthop Scand Suppl. 2003; 74(308):I, 1-30. DOI: 10.1080/000164702760300006. View

16.

Kang Y, Kim S, Khademhosseini A, Yang Y . Creation of bony microenvironment with CaP and cell-derived ECM to enhance human bone-marrow MSC behavior and delivery of BMP-2. Biomaterials. 2011; 32(26):6119-30. PMC: 3130069. DOI: 10.1016/j.biomaterials.2011.05.015. View

17.

Balemans W, Van Hul W . Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators. Dev Biol. 2002; 250(2):231-50. View

18.

Plachokova A, van den Dolder J, Stoelinga P, Jansen J . Early effect of platelet-rich plasma on bone healing in combination with an osteoconductive material in rat cranial defects. Clin Oral Implants Res. 2007; 18(2):244-51. DOI: 10.1111/j.1600-0501.2006.01327.x. View

19.

Mondrinos M, Dembzynski R, Lu L, Byrapogu V, Wootton D, Lelkes P . Porogen-based solid freeform fabrication of polycaprolactone-calcium phosphate scaffolds for tissue engineering. Biomaterials. 2006; 27(25):4399-408. DOI: 10.1016/j.biomaterials.2006.03.049. View

20.

Plachokova A, van den Dolder J, Stoelinga P, Jansen J . The bone regenerative effect of platelet-rich plasma in combination with an osteoconductive material in rat cranial defects. Clin Oral Implants Res. 2006; 17(3):305-11. DOI: 10.1111/j.1600-0501.2005.01208.x. View