In Vitro Reconstitution of the Radical S-adenosylmethionine Enzyme MqnC Involved in the Biosynthesis of Futalosine-derived Menaquinone

Overview

Journal Biochemistry

Specialty Biochemistry

Date 2013 Jun 15

PMID 23763543

Citations 13

Authors

Lisa E Cooper

Dmytro Fedoseyenko

Sameh H Abdelwahed

Soong-Hyun Kim

Tohru Dairi

Tadhg P Begley

Affiliations

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Abstract

The radical S-adenosylmethionine enzyme MqnC catalyzes conversion of dehypoxanthine futalosine (DHFL) to the unique spiro compound cyclic DHFL in the futalosine pathway for menaquinone biosynthesis. This study describes the in vitro reconstitution of [4Fe-4S] cluster-dependent MqnC activity and identifies the site of abstraction of a hydrogen atom from DHFL by the adenosyl radical.

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