Exosomes Secreted by Human Cells Transport Largely MRNA Fragments That Are Enriched in the 3'-untranslated Regions

Overview

Journal Biol Direct

Publisher Biomed Central

Specialty Biology

Date 2013 Jun 14

PMID 23758897

Citations 163

Authors

Arsen O Batagov

Igor V Kurochkin

Affiliations

Soon will be listed here.

Abstract

Unlabelled: Small secreted membrane vesicles called exosomes have recently attracted a great interest after the discovery that they transfer mRNA that can be translated into protein in recipient cells. Surprisingly, we found that for the majority of exosomal mRNAs only a fraction of their corresponding probes is detectable on the expression microarrays. Exosomal mRNA fragmentation is characterized with a specific structural pattern. The closer to the 3'-end of the transcript the fragments are localized, the larger fraction among the secreted RNAs they constitute. Since the 3'-ends of transcripts contain elements conferring subcellular localization of mRNA and are rich in miRNA-binding sites, exosomal RNA may act as competing RNA to regulate stability, localization and translation activity of mRNAs in recipient cells.

Reviewers: This article was reviewed by Neil Smalheiser and Sandor Pongor.

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