Arabidopsis ARGONAUTE7 Selects MiR390 Through Multiple Checkpoints During RISC Assembly

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2013 Jun 5

PMID 23732541

Citations 38

Authors

Yayoi Endo

Hiro-Oki Iwakawa

Yukihide Tomari

Affiliations

Soon will be listed here.

Abstract

Plant ARGONAUTE7 (AGO7) assembles RNA-induced silencing complex (RISC) specifically with miR390 and regulates the auxin-signalling pathway via production of TAS3 trans-acting siRNAs (tasiRNAs). However, how AGO7 discerns miR390 among other miRNAs remains unclear. Here, we show that the 5' adenosine of miR390 and the central region of miR390/miR390* duplex are critical for the specific interaction with AGO7. Furthermore, despite the existence of mismatches in the seed and central regions of the duplex, cleavage of the miR390* strand is required for maturation of AGO7-RISC. These findings suggest that AGO7 uses multiple checkpoints to select miR390, thereby circumventing promiscuous tasiRNA production.

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