The DNA Uptake ATPase PilF of Thermus Thermophilus: a Reexamination of the Zinc Content

Overview

Journal Extremophiles

Publisher Springer

Date 2013 May 29

PMID 23712905

Citations 4

Authors

Ralf Salzer

Martin Herzberg

Dietrich H Nies

Goran Biukovic

Gerhard Gruber

Volker Muller

Beate Averhoff

Affiliations

Soon will be listed here.

Abstract

The DNA-translocator ATPase PilF of Thermus thermophilus HB27 is a hexamer built by six identical subunits. Despite the presence of a conserved zinc-binding site in every subunit, only one zinc atom per hexamer was found. Re-examination of the zinc content of PilF purified from cells grown in complex media with different lots of yeast extract revealed six zinc atoms per hexamer. These data demonstrate that the low zinc content reported before was most likely a result of zinc depletion of the yeast extract used.

Citing Articles

Cyclic Di-GMP Binding by an Assembly ATPase (PilB2) and Control of Type IV Pilin Polymerization in the Gram-Positive Pathogen Clostridium perfringens.

Hendrick W, Orr M, Murray S, Lee V, Melville S J Bacteriol. 2017; 199(10).

PMID: 28242722 PMC: 5405213. DOI: 10.1128/JB.00034-17.

Topology and Structure/Function Correlation of Ring- and Gate-forming Domains in the Dynamic Secretin Complex of Thermus thermophilus.

Salzer R, DImprima E, Gold V, Rose I, Drechsler M, Vonck J J Biol Chem. 2016; 291(28):14448-56.

PMID: 27226590 PMC: 4938169. DOI: 10.1074/jbc.M116.724153.

Zinc and ATP binding of the hexameric AAA-ATPase PilF from Thermus thermophilus: role in complex stability, piliation, adhesion, twitching motility, and natural transformation.

Salzer R, Herzberg M, Nies D, Joos F, Rathmann B, Thielmann Y J Biol Chem. 2014; 289(44):30343-30354.

PMID: 25202014 PMC: 4215219. DOI: 10.1074/jbc.M114.598656.

Type IV pilus biogenesis, twitching motility, and DNA uptake in Thermus thermophilus: discrete roles of antagonistic ATPases PilF, PilT1, and PilT2.

Salzer R, Joos F, Averhoff B Appl Environ Microbiol. 2013; 80(2):644-52.

PMID: 24212586 PMC: 3911100. DOI: 10.1128/AEM.03218-13.

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