Lovastatin in Aspergillus Terreus: Fermented Rice Straw Extracts Interferes with Methane Production and Gene Expression in Methanobrevibacter Smithii

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2013 May 28

PMID 23710454

Citations 10

Authors

Mohammad Faseleh Jahromi

Juan Boo Liang

Yin Wan Ho

Rosfarizan Mohamad

Yong Meng Goh

Parisa Shokryazdan

James Chin

Affiliations

Soon will be listed here.

Abstract

Lovastatin, a natural byproduct of some fungi, is able to inhibit HMG-CoA (3-hydroxy-3 methyl glutaryl CoA) reductase. This is a key enzyme involved in isoprenoid synthesis and essential for cell membrane formation in methanogenic Archaea. In this paper, experiments were designed to test the hypothesis that lovastatin secreted by Aspergillus terreus in fermented rice straw extracts (FRSE) can inhibit growth and CH4 production in Methanobrevibacter smithii (a test methanogen). By HPLC analysis, 75% of the total lovastatin in FRSE was in the active hydroxyacid form, and in vitro studies confirmed that this had a stronger effect in reducing both growth and CH4 production in M. smithii compared to commercial lovastatin. Transmission electron micrographs revealed distorted morphological divisions of lovastatin- and FRSE-treated M. smithii cells, supporting its role in blocking normal cell membrane synthesis. Real-time PCR confirmed that both commercial lovastatin and FRSE increased (P < 0.01) the expression of HMG-CoA reductase gene (hmg). In addition, expressions of other gene transcripts in M. smithii. with a key involvement in methanogenesis were also affected. Experimental confirmation that CH4 production is inhibited by lovastatin in A. terreus-fermented rice straw paves the way for its evaluation as a feed additive for mitigating CH4 production in ruminants.

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References

Chinn M, Nokes S, Strobel H . Influence of process conditions on end product formation from Clostridium thermocellum 27405 in solid substrate cultivation on paper pulp sludge. Bioresour Technol. 2006; 98(11):2184-93. DOI: 10.1016/j.biortech.2006.08.033. View

Conde K, Roy S, Freake H, Newton R, Fernandez M . Atorvastatin and simvastatin have distinct effects on hydroxy methylglutaryl-CoA reductase activity and mRNA abundance in the guinea pig. Lipids. 2000; 34(12):1327-32. DOI: 10.1007/s11745-999-0485-2. View

Konrad Z, Eichler J . Lipid modification of proteins in Archaea: attachment of a mevalonic acid-based lipid moiety to the surface-layer glycoprotein of Haloferax volcanii follows protein translocation. Biochem J. 2002; 366(Pt 3):959-64. PMC: 1222828. DOI: 10.1042/BJ20020757. View

Brown M, Faust J, Goldstein J, Kaneko I, Endo A . Induction of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in human fibroblasts incubated with compactin (ML-236B), a competitive inhibitor of the reductase. J Biol Chem. 1978; 253(4):1121-8. View

Lovley D, Greening R, Ferry J . Rapidly growing rumen methanogenic organism that synthesizes coenzyme M and has a high affinity for formate. Appl Environ Microbiol. 1984; 48(1):81-7. PMC: 240316. DOI: 10.1128/aem.48.1.81-87.1984. View

Wong J, Quinn C, Gelissen I, Brown A . Endogenous 24(S),25-epoxycholesterol fine-tunes acute control of cellular cholesterol homeostasis. J Biol Chem. 2007; 283(2):700-7. DOI: 10.1074/jbc.M706416200. View

van de Donk N, Kamphuis M, Lokhorst H, Bloem A . The cholesterol lowering drug lovastatin induces cell death in myeloma plasma cells. Leukemia. 2002; 16(7):1362-71. DOI: 10.1038/sj.leu.2402501. View

Chan K, Oza A, Siu L . The statins as anticancer agents. Clin Cancer Res. 2003; 9(1):10-9. View

Mo H, Elson C . Studies of the isoprenoid-mediated inhibition of mevalonate synthesis applied to cancer chemotherapy and chemoprevention. Exp Biol Med (Maywood). 2004; 229(7):567-85. DOI: 10.1177/153537020422900701. View

10.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

11.

DeClue J, Vass W, Papageorge A, Lowy D, Willumsen B . Inhibition of cell growth by lovastatin is independent of ras function. Cancer Res. 1991; 51(2):712-7. View

12.

Balch W, Fox G, Magrum L, Woese C, WOLFE R . Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979; 43(2):260-96. PMC: 281474. DOI: 10.1128/mr.43.2.260-296.1979. View

13.

Jahromi M, Liang J, Mohamad R, Goh Y, Shokryazdan P, Wan Ho Y . Lovastatin-enriched rice straw enhances biomass quality and suppresses ruminal methanogenesis. Biomed Res Int. 2013; 2013:397934. PMC: 3581142. DOI: 10.1155/2013/397934. View

14.

Guo Y, Liu J, Lu Y, Zhu W, Denman S, McSweeney C . Effect of tea saponin on methanogenesis, microbial community structure and expression of mcrA gene, in cultures of rumen micro-organisms. Lett Appl Microbiol. 2009; 47(5):421-6. DOI: 10.1111/j.1472-765X.2008.02459.x. View

15.

Dong X, Xiao Y, Jiang X, Wang Y . Quantitative proteomic analysis revealed lovastatin-induced perturbation of cellular pathways in HL-60 cells. J Proteome Res. 2011; 10(12):5463-71. PMC: 3230662. DOI: 10.1021/pr200718p. View

16.

Hendrickson E, Haydock A, Moore B, Whitman W, Leigh J . Functionally distinct genes regulated by hydrogen limitation and growth rate in methanogenic Archaea. Proc Natl Acad Sci U S A. 2007; 104(21):8930-4. PMC: 1885605. DOI: 10.1073/pnas.0701157104. View

17.

Gao J, Weng H, Zhu D, Yuan M, Guan F, Xi Y . Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover. Bioresour Technol. 2008; 99(16):7623-9. DOI: 10.1016/j.biortech.2008.02.005. View

18.

Nakanishi M, Goldstein J, Brown M . Multivalent control of 3-hydroxy-3-methylglutaryl coenzyme A reductase. Mevalonate-derived product inhibits translation of mRNA and accelerates degradation of enzyme. J Biol Chem. 1988; 263(18):8929-37. View

19.

Clendening J, Penn L . Targeting tumor cell metabolism with statins. Oncogene. 2012; 31(48):4967-78. DOI: 10.1038/onc.2012.6. View

20.

Howe K, Sanat F, Thumser A, Coleman T, Plant N . The statin class of HMG-CoA reductase inhibitors demonstrate differential activation of the nuclear receptors PXR, CAR and FXR, as well as their downstream target genes. Xenobiotica. 2011; 41(7):519-29. DOI: 10.3109/00498254.2011.569773. View