Cocrystal Structure of the ICAP1 PTB Domain in Complex with a KRIT1 Peptide

Overview

Journal Acta Crystallogr Sect F Struct Biol Cryst Commun

Date 2013 May 23

PMID 23695561

Citations 7

Authors

WeiZhi Liu

Titus J Boggon

Affiliations

Soon will be listed here.

Abstract

Integrin cytoplasmic domain-associated protein-1 (ICAP1) is a suppressor of integrin activation and directly binds to the cytoplasmic tail of β1 integrins; its binding suppresses integrin activation by competition with talin. Krev/Rap1 interaction trapped-1 (KRIT1) releases ICAP1 suppression of integrin activation by sequestering ICAP1 away from integrin cytoplasmic tails. Here, the cocrystal structure of the PTB domain of ICAP1 in complex with a 29-amino-acid fragment (residues 170-198) of KRIT1 is presented to 1.7 Å resolution [the resolution at which 〈I/σ(I)〉 = 2.9 was 1.83 Å]. In previous studies, the structure of ICAP1 with integrin β1 was determined to 3.0 Å resolution and that of ICAP1 with the N-terminal portion of KRIT1 (residues 1-198) was determined to 2.54 Å resolution; therefore, this study provides the highest resolution structure yet of ICAP1 and allows further detailed analysis of the interaction of ICAP1 with its minimal binding region in KRIT1.

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References

McCoy A, Grosse-Kunstleve R, Storoni L, Read R . Likelihood-enhanced fast translation functions. Acta Crystallogr D Biol Crystallogr. 2005; 61(Pt 4):458-64. DOI: 10.1107/S0907444905001617. View

Murshudov G, Skubak P, Lebedev A, Pannu N, Steiner R, Nicholls R . REFMAC5 for the refinement of macromolecular crystal structures. Acta Crystallogr D Biol Crystallogr. 2011; 67(Pt 4):355-67. PMC: 3069751. DOI: 10.1107/S0907444911001314. View

Cavalcanti D, Kalani M, Martirosyan N, Eales J, Spetzler R, Preul M . Cerebral cavernous malformations: from genes to proteins to disease. J Neurosurg. 2011; 116(1):122-32. DOI: 10.3171/2011.8.JNS101241. View

Li J, Mao X, Dong L, Liu F, Tong L . Crystal structures of the BAR-PH and PTB domains of human APPL1. Structure. 2007; 15(5):525-33. DOI: 10.1016/j.str.2007.03.011. View

McNicholas S, Potterton E, Wilson K, Noble M . Presenting your structures: the CCP4mg molecular-graphics software. Acta Crystallogr D Biol Crystallogr. 2011; 67(Pt 4):386-94. PMC: 3069754. DOI: 10.1107/S0907444911007281. View

Cohen S, Morris R, Fernandez F, Ben Jelloul M, Kakaris M, Parthasarathy V . Towards complete validated models in the next generation of ARP/wARP. Acta Crystallogr D Biol Crystallogr. 2004; 60(Pt 12 Pt 1):2222-9. DOI: 10.1107/S0907444904027556. View

Emsley P, Cowtan K . Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr. 2004; 60(Pt 12 Pt 1):2126-32. DOI: 10.1107/S0907444904019158. View

Adams P, Grosse-Kunstleve R, Hung L, Ioerger T, McCoy A, Moriarty N . PHENIX: building new software for automated crystallographic structure determination. Acta Crystallogr D Biol Crystallogr. 2002; 58(Pt 11):1948-54. DOI: 10.1107/s0907444902016657. View

Fournier H, Dupe-Manet S, Bouvard D, Luton F, Degani S, Block M . Nuclear translocation of integrin cytoplasmic domain-associated protein 1 stimulates cellular proliferation. Mol Biol Cell. 2005; 16(4):1859-71. PMC: 1073667. DOI: 10.1091/mbc.e04-08-0744. View

10.

Chang D, Hoang B, Liu J, Springer T . Molecular basis for interaction between Icap1 alpha PTB domain and beta 1 integrin. J Biol Chem. 2001; 277(10):8140-5. DOI: 10.1074/jbc.M109031200. View

11.

Li X, Zhang R, Draheim K, Liu W, Calderwood D, Boggon T . Structural basis for small G protein effector interaction of Ras-related protein 1 (Rap1) and adaptor protein Krev interaction trapped 1 (KRIT1). J Biol Chem. 2012; 287(26):22317-27. PMC: 3381192. DOI: 10.1074/jbc.M112.361295. View

12.

Bouvard D, Aszodi A, Kostka G, Block M, Albiges-Rizo C, Fassler R . Defective osteoblast function in ICAP-1-deficient mice. Development. 2007; 134(14):2615-25. PMC: 2793408. DOI: 10.1242/dev.000877. View

13.

Gingras A, Liu J, Ginsberg M . Structural basis of the junctional anchorage of the cerebral cavernous malformations complex. J Cell Biol. 2012; 199(1):39-48. PMC: 3461514. DOI: 10.1083/jcb.201205109. View

14.

Brunner M, Millon-Fremillon A, Chevalier G, Nakchbandi I, Mosher D, Block M . Osteoblast mineralization requires beta1 integrin/ICAP-1-dependent fibronectin deposition. J Cell Biol. 2011; 194(2):307-22. PMC: 3144405. DOI: 10.1083/jcb.201007108. View

15.

Chen V, Arendall 3rd W, Headd J, Keedy D, Immormino R, Kapral G . MolProbity: all-atom structure validation for macromolecular crystallography. Acta Crystallogr D Biol Crystallogr. 2010; 66(Pt 1):12-21. PMC: 2803126. DOI: 10.1107/S0907444909042073. View

16.

Brutsch R, Liebler S, Wustehube J, Bartol A, Herberich S, Adam M . Integrin cytoplasmic domain-associated protein-1 attenuates sprouting angiogenesis. Circ Res. 2010; 107(5):592-601. DOI: 10.1161/CIRCRESAHA.110.217257. View

17.

Otwinowski Z, Minor W . Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 1997; 276:307-26. DOI: 10.1016/S0076-6879(97)76066-X. View

18.

Liu W, Draheim K, Zhang R, Calderwood D, Boggon T . Mechanism for KRIT1 release of ICAP1-mediated suppression of integrin activation. Mol Cell. 2013; 49(4):719-29. PMC: 3684052. DOI: 10.1016/j.molcel.2012.12.005. View

19.

Chang D, Wong C, Smith H, Liu J . ICAP-1, a novel beta1 integrin cytoplasmic domain-associated protein, binds to a conserved and functionally important NPXY sequence motif of beta1 integrin. J Cell Biol. 1997; 138(5):1149-57. PMC: 2136751. DOI: 10.1083/jcb.138.5.1149. View

20.

Holm L, Sander C . Dali/FSSP classification of three-dimensional protein folds. Nucleic Acids Res. 1997; 25(1):231-4. PMC: 146389. DOI: 10.1093/nar/25.1.231. View