Low Glibenclamide Concentrations Affect Endoplasmic Reticulum Stress in INS-1 Cells Under Glucotoxic or Glucolipotoxic Conditions

Overview

Journal Korean J Intern Med

Specialty General Medicine

Date 2013 May 18

PMID 23682228

Citations 6

Authors

Min Jeong Kwon

Hye Suk Chung

Chang Shin Yoon

Eun Ju Lee

Tae Kyun Kim

Soon Hee Lee

Kyung Soo Ko

Byoung Doo Rhee

Mi Kyung Kim

Jeong Hyun Park

Affiliations

Soon will be listed here.

Abstract

Background/aims: β-Cell apoptosis caused by increased endoplasmic reticulum (ER) stress is an important pathogenic component of type 2 diabetes mellitus. In theory, sulfonylureas, used for the treatment of diabetes, can contribute to ER stress. We assessed changes in ER stress in pancreatic β-cells under glucotoxic or glucolipotoxic conditions using low concentrations of the sulfonylurea, glibenclamide (GB).

Methods: Low concentrations of GB (10 or 100 nM) were added to INS-1 cells cultured under glucotoxic or glucolipotoxic conditions. The degree of viability, level of apoptosis and levels of markers associated with ER stress were measured.

Results: Apoptosis decreased in response to low concentrations of GB under glucolipotoxic but not glucotoxic conditions. Most ER stress markers decreased upon the addition of GB. Under glucotoxic conditions, changes in the levels of ER stress markers were not consistent. However, all decreased significantly under glucolipotoxic conditions.

Conclusions: Low concentrations of GB exerted antiapoptotic effects through the attenuation of ER stress under glucolipotoxic conditions.

Citing Articles

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