Sildenafil Inhibits the Proliferation of Cultured Human Endothelial Cells

Overview

Journal Int J Biomed Sci

Specialty General Medicine

Date 2013 May 16

PMID 23675029

Citations 3

Authors

Ali Erdogan

Doerte Wiebke Luedders

Benedikt Manuel Muenz

Christian Alexander Schaefer

Harald Tillmanns

Johannes Wiecha

Christoph Ruediger Wolfram Kuhlmann

Affiliations

Soon will be listed here.

Abstract

The proliferation of endothelial cells plays a crucial role in the development of intraplaque angiogenesis (IPA). IPA is a major source of intraplaque hemorrhage and therefore contributes to the destabilization of atherosclerotic plaques. Therefore, the aim of the present study was to examine, whether sildenafil inhibits endothelial cell growth. The proliferation of human endothelial cells derived from umbilical cord veins (HUVEC) was examined on DNA level by measurements of ((3)H)-thymidine incorporation. Cell viability was analyzed using trypan blue staining. The proliferation of cultured human endothelial cells was significantly decreased by 1 μmol/l (-48.4%) and 10 μmol/l (-89.6%) sildenafil (n=10, p<0.05). This was not a cytotoxic effect, because cell viability was only reduced at sildenafil concentrations of 50 μmol/l or greater. In addition sildenafil significantly reduced endothelial proliferation induced by bFGF (n=10, p<0.05). The presented results demonstrate an antiangiogenic effect of sildenafil that might be useful in the prevention of atherosclerotic plaque vascularization.

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