SUMO2/3 Modification of Cyclin E Contributes to the Control of Replication Origin Firing

Overview

Journal Nat Commun

Specialty Biology

Date 2013 May 16

PMID 23673635

Citations 15

Authors

Catherine Bonne-Andrea

Malik Kahli

Francisca Mechali

Jean-Marc Lemaitre

Guillaume Bossis

Olivier Coux

Affiliations

Soon will be listed here.

Abstract

The small ubiquitin-like modifier (SUMO) pathway is essential for the maintenance of genome stability. We investigated its possible involvement in the control of DNA replication during S phase by using the Xenopus cell-free system. Here we show that the SUMO pathway is critical to limit the number and, thus, the density of replication origins that are activated in early S phase. We identified cyclin E, which regulates cyclin-dependent kinase 2 (Cdk2) to trigger origin firing, as an S-phase substrate of this pathway. We show that cyclin E is dynamically and highly conjugated to SUMO2/3 on chromatin, independently of Cdk2 activity and origin activation. Moreover, cyclin E is the predominant SUMO2/3 target on chromatin in early S phase, as cyclin E depletion abolishes, while its readdition restores, the SUMO2/3 signal. Together, our data indicate that cyclin E SUMOylation is important for controlling origin firing once the cyclin E-Cdk2 complex is recruited onto replication origins.

Citing Articles

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DeSUMOylation of Apoptosis Inhibitor 5 by VP3 Supports Virus Replication.

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Revised annotation and extended characterizations of components of the SUMOylation system.

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Multisite SUMOylation restrains DNA polymerase η interactions with DNA damage sites.

Guerillon C, Smedegaard S, Hendriks I, Nielsen M, Mailand N J Biol Chem. 2020; 295(25):8350-8362.

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SUMO Protease SMT7 Modulates Ribosomal Protein L30 and Regulates Cell-Size Checkpoint Function.

Lin Y, Chung C, Chen M, Chen C, Fang S Plant Cell. 2020; 32(4):1285-1307.

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