Three-dimensional Scaffolds for Tissue Engineering Applications: Role of Porosity and Pore Size

Overview

Journal Tissue Eng Part B Rev

Specialties Anatomy & Histology
Biotechnology

Date 2013 May 16

PMID 23672709

Citations 630

Authors

Qiu Li Loh

Cleo Choong

Affiliations

Soon will be listed here.

Abstract

Tissue engineering applications commonly encompass the use of three-dimensional (3D) scaffolds to provide a suitable microenvironment for the incorporation of cells or growth factors to regenerate damaged tissues or organs. These scaffolds serve to mimic the actual in vivo microenvironment where cells interact and behave according to the mechanical cues obtained from the surrounding 3D environment. Hence, the material properties of the scaffolds are vital in determining cellular response and fate. These 3D scaffolds are generally highly porous with interconnected pore networks to facilitate nutrient and oxygen diffusion and waste removal. This review focuses on the various fabrication techniques (e.g., conventional and rapid prototyping methods) that have been employed to fabricate 3D scaffolds of different pore sizes and porosity. The different pore size and porosity measurement methods will also be discussed. Scaffolds with graded porosity have also been studied for their ability to better represent the actual in vivo situation where cells are exposed to layers of different tissues with varying properties. In addition, the ability of pore size and porosity of scaffolds to direct cellular responses and alter the mechanical properties of scaffolds will be reviewed, followed by a look at nature's own scaffold, the extracellular matrix. Overall, the limitations of current scaffold fabrication approaches for tissue engineering applications and some novel and promising alternatives will be highlighted.

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References

Chung H, Park T . Surface engineered and drug releasing pre-fabricated scaffolds for tissue engineering. Adv Drug Deliv Rev. 2007; 59(4-5):249-62. DOI: 10.1016/j.addr.2007.03.015. View

Pham Q, Sharma U, Mikos A . Electrospun poly(epsilon-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: characterization of scaffolds and measurement of cellular infiltration. Biomacromolecules. 2006; 7(10):2796-805. DOI: 10.1021/bm060680j. View

Sell S, Barnes C, Simpson D, Bowlin G . Scaffold permeability as a means to determine fiber diameter and pore size of electrospun fibrinogen. J Biomed Mater Res A. 2007; 85(1):115-26. DOI: 10.1002/jbm.a.31556. View

Bollheimer L, Wrede C, Rockmann F, Ottinger I, Scholmerich J, Buettner R . Glucagon production of the rat insulinoma cell line INS-1-A quantitative comparison with primary rat pancreatic islets. Biochem Biophys Res Commun. 2005; 330(1):327-32. DOI: 10.1016/j.bbrc.2005.01.168. View

Pereverzev A, Vajna R, Pfitzer G, Hescheler J, Klockner U, Schneider T . Reduction of insulin secretion in the insulinoma cell line INS-1 by overexpression of a Ca(v)2.3 (alpha1E) calcium channel antisense cassette. Eur J Endocrinol. 2002; 146(6):881-9. DOI: 10.1530/eje.0.1460881. View

Wu C, Luo Y, Cuniberti G, Xiao Y, Gelinsky M . Three-dimensional printing of hierarchical and tough mesoporous bioactive glass scaffolds with a controllable pore architecture, excellent mechanical strength and mineralization ability. Acta Biomater. 2011; 7(6):2644-50. DOI: 10.1016/j.actbio.2011.03.009. View

Li C, Vepari C, Jin H, Kim H, Kaplan D . Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials. 2006; 27(16):3115-24. DOI: 10.1016/j.biomaterials.2006.01.022. View

Damien E, Hing K, Saeed S, Revell P . A preliminary study on the enhancement of the osteointegration of a novel synthetic hydroxyapatite scaffold in vivo. J Biomed Mater Res A. 2003; 66(2):241-6. DOI: 10.1002/jbm.a.10564. View

Luu Y, Kim K, Hsiao B, Chu B, Hadjiargyrou M . Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA-PEG block copolymers. J Control Release. 2003; 89(2):341-53. DOI: 10.1016/s0168-3659(03)00097-x. View

10.

Zimmermann U, Mimietz S, Zimmermann H, Hillgartner M, Schneider H, Ludwig J . Hydrogel-based non-autologous cell and tissue therapy. Biotechniques. 2000; 29(3):564-72, 574, 576 passim. DOI: 10.2144/00293rv01. View

11.

Goulet R, Goldstein S, Ciarelli M, Kuhn J, Brown M, Feldkamp L . The relationship between the structural and orthogonal compressive properties of trabecular bone. J Biomech. 1994; 27(4):375-89. DOI: 10.1016/0021-9290(94)90014-0. View

12.

Luo Y, Shoichet M . A photolabile hydrogel for guided three-dimensional cell growth and migration. Nat Mater. 2004; 3(4):249-53. DOI: 10.1038/nmat1092. View

13.

Wang M, Pei H, Zhang L, Guan L, Zhang R, Jia Y . Hepatogenesis of adipose-derived stem cells on poly-lactide-co-glycolide scaffolds: in vitro and in vivo studies. Tissue Eng Part C Methods. 2010; 16(5):1041-50. DOI: 10.1089/ten.TEC.2009.0244. View

14.

Tan K, Chua C, Leong K, Cheah C, Cheang P, Abu Bakar M . Scaffold development using selective laser sintering of polyetheretherketone-hydroxyapatite biocomposite blends. Biomaterials. 2003; 24(18):3115-23. DOI: 10.1016/s0142-9612(03)00131-5. View

15.

Kloxin A, Tibbitt M, Kasko A, Fairbairn J, Anseth K . Tunable hydrogels for external manipulation of cellular microenvironments through controlled photodegradation. Adv Mater. 2010; 22(1):61-6. PMC: 4548878. DOI: 10.1002/adma.200900917. View

16.

Oliviero O, Ventre M, Netti P . Functional porous hydrogels to study angiogenesis under the effect of controlled release of vascular endothelial growth factor. Acta Biomater. 2012; 8(9):3294-301. DOI: 10.1016/j.actbio.2012.05.019. View

17.

Constantinidis I, Grant S, Celper S, Gauffin-Holmberg I, Agering K, Oca-Cossio J . Non-invasive evaluation of alginate/poly-l-lysine/alginate microcapsules by magnetic resonance microscopy. Biomaterials. 2007; 28(15):2438-45. PMC: 2083257. DOI: 10.1016/j.biomaterials.2007.01.012. View

18.

Hohmeier H, Newgard C . Cell lines derived from pancreatic islets. Mol Cell Endocrinol. 2004; 228(1-2):121-8. DOI: 10.1016/j.mce.2004.04.017. View

19.

Karageorgiou V, Kaplan D . Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2005; 26(27):5474-91. DOI: 10.1016/j.biomaterials.2005.02.002. View

20.

Tsang V, Bhatia S . Three-dimensional tissue fabrication. Adv Drug Deliv Rev. 2004; 56(11):1635-47. DOI: 10.1016/j.addr.2004.05.001. View