Saliva Improves Streptococcus Mitis Protective Effect on Human Gingival Fibroblasts in Presence of 2-hydroxyethyl-methacrylate

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2013 May 15

PMID 23670604

Citations 4

Authors

Mara Di Giulio

Viviana di Giacomo

Emanuela Di Campli

Soraya Di Bartolomeo

Susi Zara

Guido Pasquantonio

Amelia Cataldi

Luigina Cellini

Affiliations

Soon will be listed here.

Abstract

This study aimed to investigate the effect of saliva on Streptococcus mitis free cells and on S. mitis/human gingival fibroblasts (HGFs) co-culture model, in presence of 2-hydroxyethyl-methacrylate (HEMA). The bacterial aggregation both in the planktonic phase and on HGFs, as well as the apoptotic and necrotic eukaryotic cells amount were analyzed, in presence of saliva and/or HEMA. The aggregation test revealed a significant saliva aggregation effect on S. mitis strains compared to the untreated sample. No significant differences were recorded in the amount of culturable bacteria in all studied conditions; however, from microscopy images, the saliva/HEMA combining effect induced a significant bacterial aggregation and adhesion on HGFs. HEMA treatment decreased viable eukaryotic cell number with a parallel increment of necrotic cells, but when saliva was added to the co-culture, the viable cells percentage increased to a value comparable to the control sample.

Citing Articles

Enterococcus hirae biofilm formation on hospital material surfaces and effect of new biocides.

Di Lodovico S, Cataldi V, Di Campli E, Ancarani E, Cellini L, Di Giulio M Environ Health Prev Med. 2017; 22(1):63.

PMID: 29165147 PMC: 5664585. DOI: 10.1186/s12199-017-0670-3.

Adhesion of human gingival fibroblasts/Streptococcus mitis co-culture on the nanocomposite system Chitlac-nAg.

Cataldi A, Gallorini M, Di Giulio M, Guarnieri S, Mariggio M, Traini T J Mater Sci Mater Med. 2016; 27(5):88.

PMID: 26970770 PMC: 4789204. DOI: 10.1007/s10856-016-5701-x.

NF-kB mediated down-regulation of collagen synthesis upon HEMA (2-hydroxyethyl methacrylate) treatment of primary human gingival fibroblast/Streptococcus mutans co-cultured cells.

Grande R, Pacella S, Di Giulio M, Rapino M, Di Valerio V, Cellini L Clin Oral Investig. 2014; 19(4):841-9.

PMID: 25200938 PMC: 4429030. DOI: 10.1007/s00784-014-1304-4.

Biological responses of human gingival fibroblasts (HGFs) in an innovative co-culture model with Streptococcus mitis to thermosets coated with a silver polysaccharide antimicrobial system.

Sancilio S, di Giacomo V, Di Giulio M, Gallorini M, Marsich E, Travan A PLoS One. 2014; 9(5):e96520.

PMID: 24806464 PMC: 4013038. DOI: 10.1371/journal.pone.0096520.

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