Prdm14 Promotes Germline Fate and Naive Pluripotency by Repressing FGF Signalling and DNA Methylation

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2013 May 15

PMID 23670199

Citations 85

Authors

Nils Grabole

Julia Tischler

Jamie A Hackett

Shinseog Kim

Fuchou Tang

Harry G Leitch

Erna Magnusdottir

M Azim Surani

Affiliations

Soon will be listed here.

Abstract

Primordial germ cells (PGCs) and somatic cells originate from postimplantation epiblast cells in mice. As pluripotency is lost upon differentiation of somatic lineages, a naive epigenome and the pluripotency network are re-established during PGC development. Here we demonstrate that Prdm14 contributes not only to PGC specification, but also to naive pluripotency in embryonic stem (ES) cells by repressing the DNA methylation machinery and fibroblast growth factor (FGF) signalling. This indicates a critical role for Prdm14 in programming PGCs and promoting pluripotency in ES cells.

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