Development of TaqMan-based QPCR Method for Detection of Caprine Arthritis-encephalitis Virus (CAEV) Infection

Overview

Journal Arch Virol

Specialty Microbiology

Date 2013 May 15

PMID 23670072

Citations 5

Authors

Yi Li

Fengjuan Zhou

Xia Li

Jianhua Wang

Xiangping Zhao

Jinhai Huang

Affiliations

Soon will be listed here.

Abstract

A specific and sensitive two-step TaqMan real-time PCR has been developed for rapid diagnosis of caprine arthritis-encephalitis virus (CAEV) infection by using a set of specific primers and a TaqMan probe targeting a highly conserved region within the gene encoding the viral capsid protein (CA). The assay successfully detected CAEV proviral DNA in total DNA extracts originating from cell culture, whole blood samples and isolated PBMCs, with a lower detection limit of 10(2) copies and a linear dynamic range of 10(5) to 10(10) copies/ml. There was no cross-reaction with other animal viruses (e.g., goat pox virus, bovine leukemia virus, bovine mucosal disease virus, swine influenza virus and Nipah virus). When applied in parallel with serological AGID and conventional PCR for detection of CAEV in field samples, this assay exhibited a higher sensitivity than these traditional methods, and 7.8 % of the 308 specimens collected in the Shanxi and Tianjin regions of China from 1993 to 2011 were found to be positive. Thus, the TaqMan qPCR assay provides a fast, specific and sensitive means for detecting CAEV proviral DNA in goat specimens and should be useful for large-scale detection in eradication programs and epidemiological studies.

Citing Articles

Detection and Phylogenetic Analysis of Caprine Arthritis Encephalitis Virus Using TaqMan-based qPCR in Eastern China.

Tian Y, Zhang H, Zhang Y, Zhang X, Guan Z, Zhang J Vet Sci. 2024; 11(3).

PMID: 38535872 PMC: 10975075. DOI: 10.3390/vetsci11030138.

The dUTPase of caprine arthritis-encephalitis virus negatively regulates interferon signaling pathway.

Shi J, Li X, Zhu M, Chi H, Song Y, Wang J Iran J Vet Res. 2021; 22(3):209-216.

PMID: 34777521 PMC: 8573393. DOI: 10.22099/ijvr.2021.38240.5568.

The Vif protein of caprine arthritis encephalitis virus inhibits interferon production.

Fu Y, Lu D, Su Y, Chi H, Wang J, Huang J Arch Virol. 2020; 165(7):1557-1567.

PMID: 32356187 DOI: 10.1007/s00705-020-04637-z.

Advances in diagnosis of respiratory diseases of small ruminants.

Chakraborty S, Kumar A, Tiwari R, Rahal A, Malik Y, Dhama K Vet Med Int. 2014; 2014:508304.

PMID: 25028620 PMC: 4082846. DOI: 10.1155/2014/508304.

Development and validation of TaqMan probe based real time PCR assays for the specific detection of genotype A and B small ruminant lentivirus strains.

Kuhar U, Barlic-Maganja D, Grom J BMC Vet Res. 2013; 9:172.

PMID: 24004524 PMC: 3766269. DOI: 10.1186/1746-6148-9-172.

References

Grego E, Profiti M, Giammarioli M, Giannino L, Rutili D, Woodall C . Genetic heterogeneity of small ruminant lentiviruses involves immunodominant epitope of capsid antigen and affects sensitivity of single-strain-based immunoassay. Clin Diagn Lab Immunol. 2002; 9(4):828-32. PMC: 120019. DOI: 10.1128/cdli.9.4.828-832.2002. View

Synge B, Ritchie C . Elimination of small ruminant lentivirus infection from sheep flocks and goat herds aided by health schemes in Great Britain. Vet Rec. 2011; 167(19):739-43. DOI: 10.1136/vr.c5853. View

Rosati S, Profiti M, Lorenzetti R, Bandecchi P, Mannelli A, Ortoffi M . Development of recombinant capsid antigen/transmembrane epitope fusion proteins for serological diagnosis of animal lentivirus infections. J Virol Methods. 2004; 121(1):73-8. DOI: 10.1016/j.jviromet.2004.06.001. View

Rowe J, East N, Franti C, Thurmond M, Pedersen N, Theilen G . Risk factors associated with the incidence of seroconversion to caprine arthritis-encephalitis virus in goats on California dairies. Am J Vet Res. 1992; 53(12):2396-403. View

Konishi M, Tsuduku S, Haritani M, Murakami K, Tsuboi T, Kobayashi C . An epidemic of caprine arthritis encephalitis in Japan: isolation of the virus. J Vet Med Sci. 2004; 66(8):911-7. DOI: 10.1292/jvms.66.911. View

Martins G, Penna B, Hamond C, Leite R, Silva A, Ferreira A . Leptospirosis as the most frequent infectious disease impairing productivity in small ruminants in Rio de Janeiro, Brazil. Trop Anim Health Prod. 2011; 44(4):773-7. DOI: 10.1007/s11250-011-9964-4. View

Zanoni R, Nauta I, Pauli U, Peterhans E . Expression in Escherichia coli and sequencing of the coding region for the capsid protein of Dutch maedi-visna virus strain ZZV 1050: application of recombinant protein in enzyme-linked immunosorbent assay for the detection of caprine and ovine.... J Clin Microbiol. 1991; 29(7):1290-4. PMC: 270102. DOI: 10.1128/jcm.29.7.1290-1294.1991. View

Gorrell M, Brandon M, Sheffer D, Adams R, Narayan O . Ovine lentivirus is macrophagetropic and does not replicate productively in T lymphocytes. J Virol. 1992; 66(5):2679-88. PMC: 241022. DOI: 10.1128/JVI.66.5.2679-2688.1992. View

Rosati S, Pittau M, Tolari F, Erre G, Kwang J . Genetic and antigenic characterization of caev (caprine arthritis-encephalitis virus) recombinant transmembrane protein. Vet Microbiol. 1995; 45(4):363-70. DOI: 10.1016/0378-1135(94)00138-m. View

10.

Luengo C, Sanchez A, Corrales J, Fernandez C, Contreras A . Influence of intramammary infection and non-infection factors on somatic cell counts in dairy goats. J Dairy Res. 2004; 71(2):169-74. DOI: 10.1017/s0022029904000019. View

11.

Peterhans E, Greenland T, Badiola J, Harkiss G, Bertoni G, Amorena B . Routes of transmission and consequences of small ruminant lentiviruses (SRLVs) infection and eradication schemes. Vet Res. 2004; 35(3):257-74. DOI: 10.1051/vetres:2004014. View

12.

de Andres D, Klein D, Watt N, Berriatua E, Torsteinsdottir S, Blacklaws B . Diagnostic tests for small ruminant lentiviruses. Vet Microbiol. 2005; 107(1-2):49-62. DOI: 10.1016/j.vetmic.2005.01.012. View

13.

Cork L, Hadlow W, Crawford T, GORHAM J, Piper R . Infectious leukoencephalomyelitis of young goats. J Infect Dis. 1974; 129(2):134-41. DOI: 10.1093/infdis/129.2.134. View

14.

Gjerset B, Jonassen C, Rimstad E . Natural transmission and comparative analysis of small ruminant lentiviruses in the Norwegian sheep and goat populations. Virus Res. 2007; 125(2):153-61. DOI: 10.1016/j.virusres.2006.12.014. View

15.

Crespo Leon F, Gutierrez Diez F, Rodriguez Ferri F, Leon Vizcaino L, Cuello Gijon F, Gimeno E . The translation into Spanish of the OIE Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees): problems, solutions and conclusions. Rev Sci Tech. 2006; 24(3):1095-104. View

16.

Eltahir Y, Dovas C, Papanastassopoulou M, Koumbati M, Giadinis N, Verghese-Nikolakaki S . Development of a semi-nested PCR using degenerate primers for the generic detection of small ruminant lentivirus proviral DNA. J Virol Methods. 2006; 135(2):240-6. DOI: 10.1016/j.jviromet.2006.03.010. View

17.

Narayan O, Kennedy-Stoskopf S, Sheffer D, Griffin D, Clements J . Activation of caprine arthritis-encephalitis virus expression during maturation of monocytes to macrophages. Infect Immun. 1983; 41(1):67-73. PMC: 264744. DOI: 10.1128/iai.41.1.67-73.1983. View

18.

Oem J, Chung J, Byun J, Kim H, Kwak D, Jung B . Large-scale serological survey of caprine arthritis-encephalitis virus (CAEV) in Korean black goats (Capra hircus aegagrus). J Vet Med Sci. 2012; 74(12):1657-9. DOI: 10.1292/jvms.12-0103. View

19.

Sanchez A, Contreras A, Corrales J, Marco J . Relationships between infection with caprine arthritis encephalitis virus, intramammary bacterial infection and somatic cell counts in dairy goats. Vet Rec. 2001; 148(23):711-4. DOI: 10.1136/vr.148.23.711. View

20.

Brajon G, Mandas D, Liciardi M, Taccori F, Meloni M, Corrias F . Development and Field Testing of a Real-Time PCR Assay for Caprine Arthritis-Encephalitis-Virus (CAEV). Open Virol J. 2012; 6:82-90. PMC: 3414719. DOI: 10.2174/1874357901206010082. View