Transcriptional Analysis of Oligosaccharide Utilization by Bifidobacterium Lactis Bl-04

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2013 May 14

PMID 23663691

Citations 33

Authors

Joakim M Andersen

Rodolphe Barrangou

Maher Abou Hachem

Sampo J Lahtinen

Yong Jun Goh

Birte Svensson

Todd R Klaenhammer

Affiliations

Soon will be listed here.

Abstract

Background: Probiotic bifidobacteria in combination with prebiotic carbohydrates have documented positive effects on human health regarding gastrointestinal disorders and improved immunity, however the selective routes of uptake remain unknown for most candidate prebiotics. The differential transcriptomes of Bifidobacterium animalis subsp. lactis Bl-04, induced by 11 potential prebiotic oligosaccharides were analyzed to identify the genetic loci involved in the uptake and catabolism of α- and β-linked hexoses, and β-xylosides.

Results: The overall transcriptome was modulated dependent on the type of glycoside (galactosides, glucosides or xylosides) utilized. Carbohydrate transporters of the major facilitator superfamily (induced by gentiobiose and β-galacto-oligosaccharides (GOS)) and ATP-binding cassette (ABC) transporters (upregulated by cellobiose, GOS, isomaltose, maltotriose, melibiose, panose, raffinose, stachyose, xylobiose and β-xylo-oligosaccharides) were differentially upregulated, together with glycoside hydrolases from families 1, 2, 13, 36, 42, 43 and 77. Sequence analysis of the identified solute-binding proteins that determine the specificity of ABC transporters revealed similarities in the breadth and selectivity of prebiotic utilization by bifidobacteria.

Conclusion: This study identified the differential gene expression for utilization of potential prebiotics highlighting the extensive capabilities of Bifidobacterium lactis Bl-04 to utilize oligosaccharides. Results provide insights into the ability of this probiotic microbe to utilize indigestible carbohydrates in the human gastrointestinal tract.

Citing Articles

Determinants of raffinose family oligosaccharide use in species.

Basu A, Adams A, Degnan P, Vanderpool C J Bacteriol. 2024; 206(10):e0023524.

PMID: 39330254 PMC: 11501099. DOI: 10.1128/jb.00235-24.

Transcriptomic and metabolomic analysis of prebiotics utilization by Bifidobacterium animalis.

Liu T, Bai H, Wang S, Gong W, Wang Z World J Microbiol Biotechnol. 2024; 40(8):257.

PMID: 38937374 DOI: 10.1007/s11274-024-04061-4.

Determinants of raffinose family oligosaccharide use in species.

Basu A, Adams A, Degnan P, Vanderpool C bioRxiv. 2024; .

PMID: 38895307 PMC: 11185731. DOI: 10.1101/2024.06.07.597959.

Whole-genome resequencing and transcriptional profiling association analysis revealed the intraspecies difference response to oligosaccharides utilization in subsp. .

Lan Z, Zhang X, Xu M, Kong J, Zuo X, Wang Y Front Microbiol. 2024; 15:1375384.

PMID: 38659979 PMC: 11041377. DOI: 10.3389/fmicb.2024.1375384.

Journey of the Probiotic Bacteria: Survival of the Fittest.

Andrade Mendonca A, Pinto-Neto W, da Paixao G, da Silva Santos D, de Morais Jr M, de Souza R Microorganisms. 2023; 11(1).

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