Phospholipid Composition and Electric Charge in Healthy and Cancerous Parts of Human Kidneys

Overview

Journal J Membr Biol

Date 2013 May 8

PMID 23649039

Citations 20

Authors

Barbara Szachowicz-Petelska

Izabela Dobrzynska

Marta Skrodzka

Barbara Darewicz

Zbigniew A Figaszewski

Jacek Kudelski

Affiliations

Soon will be listed here.

Abstract

Phospholipids are ubiquitous in nature and are essential for the lipid bilayer of cell membranes. Their structural and functional properties are pivotal for the survival of the cell. In this study the phospholipids of healthy and cancerous human renal tissues from the same patients are compared with special reference to the electric charge of the membrane. A simple and highly effective normal-phase method is described for analyzing phospholipids content. This work is focused on changes of phospholipids content (PtdIns, phosphatidylinositol; PtdSer, phosphatidylserine; PtdEtn, phosphatidylethanoloamine; PtdCho, phosphatidylcholine) in cell membranes of renal cancer of pT1 stage, G2 grade, without metastasis. Surface charge density of healthy and cancerous human renal tissues was measured by electrophoresis. The measurements were carried out at various pH of solution. Depending on the surface charge density as a function of pH, acidic (C(TA)) and basic (C(TB)) functional group concentrations and their average association constants with hydrogen (K(AH)) or hydroxyl (K(BOH)) ions were evaluated. The process of cancer transformation was accompanied by an increase in total amount of phospholipids as well as an increase in C(TA) and K(BOH), whereas K(AH) and C(TB) were decreased compared with unchanged tumor cells.

Citing Articles

Adipocytes reprogram cancer cell metabolism by diverting glucose towards glycerol-3-phosphate thereby promoting metastasis.

Mukherjee A, Bezwada D, Greco F, Zandbergen M, Shen T, Chiang C Nat Metab. 2023; 5(9):1563-1577.

PMID: 37653041 DOI: 10.1038/s42255-023-00879-8.

Perturbing plasma membrane lipid: a new paradigm for tumor nanotherapeutics.

Fei W, Yan J, Wu X, Yang S, Zhang X, Wang R Theranostics. 2023; 13(8):2471-2491.

PMID: 37215569 PMC: 10196822. DOI: 10.7150/thno.82189.

Nano-bio surface interactions, cellular internalisation in cancer cells and e-data portals of nanomaterials: A review.

Yadav R, Chaudhary A IET Nanobiotechnol. 2021; 15(6):519-531.

PMID: 34694743 PMC: 8675851. DOI: 10.1049/nbt2.12040.

Monoethanolamine-induced glucose deprivation promotes apoptosis through metabolic rewiring in prostate cancer.

Garlapati C, Joshi S, Turaga R, Mishra M, Reid M, Kapoor S Theranostics. 2021; 11(18):9089-9106.

PMID: 34522228 PMC: 8419048. DOI: 10.7150/thno.62724.

Fabrication and electromechanical characterization of freestanding asymmetric membranes.

Liu P, Zabala-Ferrera O, Beltramo P Biophys J. 2021; 120(9):1755-1764.

PMID: 33675759 PMC: 8204216. DOI: 10.1016/j.bpj.2021.02.036.

References

Semenza G . Targeting HIF-1 for cancer therapy. Nat Rev Cancer. 2003; 3(10):721-32. DOI: 10.1038/nrc1187. View

Zhao J, Zhou Q, Wiedmer T, Sims P . Level of expression of phospholipid scramblase regulates induced movement of phosphatidylserine to the cell surface. J Biol Chem. 1998; 273(12):6603-6. DOI: 10.1074/jbc.273.12.6603. View

Dobrzynska I, Szachowicz-Petelska B, Sulkowski S, Figaszewski Z . Changes in electric charge and phospholipids composition in human colorectal cancer cells. Mol Cell Biochem. 2005; 276(1-2):113-9. DOI: 10.1007/s11010-005-3557-3. View

Evans W . Fractionation of liver plasma membranes prepared by zonal centrifugation. Biochem J. 1970; 116(5):833-42. PMC: 1185506. DOI: 10.1042/bj1160833. View

Ran S, Thorpe P . Phosphatidylserine is a marker of tumor vasculature and a potential target for cancer imaging and therapy. Int J Radiat Oncol Biol Phys. 2002; 54(5):1479-84. DOI: 10.1016/s0360-3016(02)03928-7. View

Hoffmann K, Blaudszun J, Brunken C, Hopker W, Tauber R, Steinhart H . Lipid class distribution of fatty acids including conjugated linoleic acids in healthy and cancerous parts of human kidneys. Lipids. 2005; 40(10):1057-62. DOI: 10.1007/s11745-005-1469-y. View

Inagaki T, Bhayani S, Allaf M, Ong A, Rha K, Petresior D . Tumor capacitance: electrical measurements of renal neoplasia. J Urol. 2004; 172(2):454-7. DOI: 10.1097/01.ju.0000129300.61874.17. View

Hemminki K, Jiang Y, Ma X, Yang K, Egevad L, Lindblad P . Molecular epidemiology of VHL gene mutations in renal cell carcinoma patients: relation to dietary and other factors. Carcinogenesis. 2002; 23(5):809-15. DOI: 10.1093/carcin/23.5.809. View

Dobrzynska I, Skrzydlewska E, Figaszewski Z . Parameters characterizing acid-base equilibria between cell membrane and solution and their application to monitoring the effect of various factors on the membrane. Bioelectrochemistry. 2006; 69(2):142-7. DOI: 10.1016/j.bioelechem.2006.01.004. View

10.

White S, Ladokhin A, Jayasinghe S, Hristova K . How membranes shape protein structure. J Biol Chem. 2001; 276(35):32395-8. DOI: 10.1074/jbc.R100008200. View

11.

Lee T . Biosynthesis and possible biological functions of plasmalogens. Biochim Biophys Acta. 1998; 1394(2-3):129-45. DOI: 10.1016/s0005-2760(98)00107-6. View

12.

Szachowicz-Petelska B, Dobrzynska I, Figaszewski Z, Sulkowski S . Changes in physico-chemical properties of human large intestine tumour cells membrane. Mol Cell Biochem. 2002; 238(1-2):41-7. DOI: 10.1023/a:1019946718876. View

13.

Turner K, Moore J, Jones A, Taylor C, Cuthbert-Heavens D, Han C . Expression of hypoxia-inducible factors in human renal cancer: relationship to angiogenesis and to the von Hippel-Lindau gene mutation. Cancer Res. 2002; 62(10):2957-61. View

14.

Lunt S, Vander Heiden M . Aerobic glycolysis: meeting the metabolic requirements of cell proliferation. Annu Rev Cell Dev Biol. 2011; 27:441-64. DOI: 10.1146/annurev-cellbio-092910-154237. View

15.

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

16.

Ran S, Downes A, Thorpe P . Increased exposure of anionic phospholipids on the surface of tumor blood vessels. Cancer Res. 2002; 62(21):6132-40. View

17.

Podo F . Tumour phospholipid metabolism. NMR Biomed. 2000; 12(7):413-39. DOI: 10.1002/(sici)1099-1492(199911)12:7<413::aid-nbm587>3.0.co;2-u. View

18.

Nicol B, Prasad S . Sialic acid changes in Dalton's lymphoma-bearing mice after cyclophosphamide and cisplatin treatment. Braz J Med Biol Res. 2002; 35(5):549-53. DOI: 10.1590/s0100-879x2002000500006. View

19.

Hannun Y, Luberto C, Argraves K . Enzymes of sphingolipid metabolism: from modular to integrative signaling. Biochemistry. 2001; 40(16):4893-903. DOI: 10.1021/bi002836k. View

20.

Ruiz-Cabello J, Cohen J . Phospholipid metabolites as indicators of cancer cell function. NMR Biomed. 1992; 5(5):226-33. DOI: 10.1002/nbm.1940050506. View