LKB1 Controls Human Bronchial Epithelial Morphogenesis Through P114RhoGEF-dependent RhoA Activation

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2013 May 8

PMID 23648482

Citations 31

Authors

Xiaojian Xu

Dan Jin

Joanne Durgan

Alan Hall

Affiliations

Soon will be listed here.

Abstract

LKB1 is a Ser/Thr kinase that plays an important role in controlling both energy metabolism and cell polarity in metazoan organisms. LKB1 is also a tumor suppressor, and homozygous, inactivating mutations are found in a wide range of human cancers. In lung cancer, inactivating mutations are found in 10 to 50% of cases, but the consequences of functional loss in this context are poorly understood. We report here that LKB1 is required for the maturation of apical junctions in the human bronchial epithelial cell line 16HBE14o- (16HBE). This activity is dependent on an interaction with the Rho guanine nucleotide exchange factor p114RhoGEF but is independent of LKB1 kinase activity. Together, LKB1 and p114RhoGEF control RhoA activity in these cells to promote apical junction assembly.

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