Epithelial Cell Proliferation Arrest Induced by Lactate and Acetate from Lactobacillus Casei and Bifidobacterium Breve

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 May 7

PMID 23646174

Citations 38

Authors

Takahiro Matsuki

Thierry Pedron

Beatrice Regnault

Celine Mulet

Taeko Hara

Philippe J Sansonetti

Affiliations

Soon will be listed here.

Abstract

In an attempt to identify and characterize how symbiotic bacteria of the gut microbiota affect the molecular and cellular mechanisms of epithelial homeostasis, intestinal epithelial cells were co-cultured with either Lactobacillus or Bifidobacterium as bona fide symbionts to examine potential gene modulations. In addition to genes involved in the innate immune response, genes encoding check-point molecules controlling the cell cycle were among the most modulated in the course of these interactions. In the m-ICcl2 murine cell line, genes encoding cyclin E1 and cyclin D1 were strongly down regulated by L. casei and B. breve respectively. Cell proliferation arrest was accordingly confirmed. Short chain fatty acids (SCFA) were the effectors of this modulation, alone or in conjunction with the acidic pH they generated. These results demonstrate that the production of SCFAs, a characteristic of these symbiotic microorganisms, is potentially an essential regulatory effector of epithelial proliferation in the gut.

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