Detection of Spliced MRNA from Human Bocavirus 1 in Clinical Samples from Children with Respiratory Tract Infections

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2013 May 1

PMID 23628409

Citations 22

Authors

Andreas Christensen

Henrik Dollner

Lars Hosoien Skanke

Sidsel Krokstad

Nina Moe

Svein Arne Nordbo

Affiliations

Soon will be listed here.

Abstract

Human bocavirus 1 (HBoV1) is a parvovirus associated with respiratory tract infections (RTIs) in children, but a causal relation has not yet been confirmed. To develop a qualitative reverse transcription PCR to detect spliced mRNA from HBoV1 and to determine whether HBoV1 mRNA correlated better with RTIs than did HBoV1 DNA, we used samples from HBoV1 DNA-positive children, with and without RTIs, to evaluate the test. A real-time reverse transcription PCR, targeting 2 alternatively spliced mRNAs, was developed. HBoV1 mRNA was detected in nasopharyngeal aspirates from 33 (25%) of 133 children with RTIs but in none of 28 controls (p<0.001). The analytical sensitivity and specificity of the test were good. Our data support the hypothesis that HBoV1 may cause RTIs, and we propose that HBoV1 mRNA could be used with benefit, instead of HBoV1 DNA, as a diagnostic target.

Citing Articles

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