N-terminal Acetylation by NatC is Not a General Determinant for Substrate Subcellular Localization in Saccharomyces Cerevisiae

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Apr 25

PMID 23613772

Citations 15

Authors

Henriette Aksnes

Camilla Osberg

Thomas Arnesen

Affiliations

Soon will be listed here.

Abstract

N-terminal acetylation has been suggested to play a role in the subcellular targeting of proteins, in particular those acetylated by the N-terminal acetyltransferase complex NatC. Based on previous positional proteomics data revealing N-terminal acetylation status and the predicted NAT substrate classes, we selected 13 suitable NatC substrates for subcellular localization studies in Saccharomyces cerevisiae. Fluorescence microscopy analysis of GFP-tagged candidates in the presence or absence of the NatC catalytic subunit Naa30 (Mak3) revealed unaltered localization patterns for all 13 candidates, thus arguing against a general role for the N-terminal acetyl group as a localization determinant. Furthermore, all organelle-localized substrates indicated undisrupted structures, thus suggesting that absence of NatC acetylation does not have a vast effect on organelle morphology in yeast.

Citing Articles

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