Cholesterol Dependent Uptake and Interaction of Doxorubicin in Mcf-7 Breast Cancer Cells

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2013 Apr 18

PMID 23591847

Citations 14

Authors

Petra Weber

Michael Wagner

Herbert Schneckenburger

Affiliations

Soon will be listed here.

Abstract

Methods of fluorescence spectroscopy and microscopy-including intensity and lifetime (FLIM) images-are used to examine uptake, intracellular location and interaction of the chemotherapeutic drug doxorubicin in MCF-7 human breast cancer cells as a function of cholesterol content. By comparing cells with natural and decreased cholesterol levels after 2 h or 24 h incubation with doxorubicin, we observed that higher fluorescence intensities and possibly shortened fluorescence lifetimes-reflecting increased uptake of the drug and more pronounced drug response-are concomitant with higher membrane fluidity.

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