Levels of Supramolecular Chirality of Polyglutamine Aggregates Revealed by Vibrational Circular Dichroism

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2013 Apr 16

PMID 23583713

Citations 10

Authors

Dmitry Kurouski

Karunakar Kar

Ronald Wetzel

Rina K Dukor

Igor K Lednev

Laurence A Nafie

Affiliations

Soon will be listed here.

Abstract

Polyglutamine (PolyQ) aggregates are a hallmark of several severe neurodegenerative diseases, expanded CAG-repeat diseases in which inheritance of an expanded polyQ sequence above a pathological threshold is associated with a high risk of disease. Application of vibrational circular dichroism (VCD) reveals that these PolyQ fibril aggregates exhibit a chiral supramolecular organization that is distinct from the supramolecular organization of previously observed amyloid fibrils. PolyQ fibrils grown from monomers with Q repeats 35 and above (Q≥35) exhibit approximately 10-fold enhancement of the same VCD spectrum compared to the already enhanced VCD of fibrils formed from Q repeats 30 and below (Q≤30).

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