Comparison of in Vivo Optical Systems for Bioluminescence and Fluorescence Imaging

Overview

Journal J Fluoresc

Specialties Biophysics
Chemistry

Date 2013 Apr 13

PMID 23579930

Citations 18

Authors

Steven K Cool

Koen Breyne

Evelyne Meyer

Stefaan C De Smedt

Niek N Sanders

Affiliations

Soon will be listed here.

Abstract

In vivo optical imaging has become a popular tool in animal laboratories. Currently, many in vivo optical imaging systems are available on the market, which often makes it difficult for research groups to decide which system fits their needs best. In this work we compared different commercially available systems, which can measure both bioluminescent and fluorescent light. The systems were tested for their bioluminescent and fluorescent sensitivity both in vitro and in vivo. The IVIS Lumina II was found to be most sensitive for bioluminescence imaging, with the Photon Imager a close second. Contrary, the Kodak system was, in vitro, the most sensitive system for fluorescence imaging. In vivo, the fluorescence sensitivity of the systems was similar. Finally, we examined the added value of spectral unmixing algorithms for in vivo optical imaging and demonstrated that spectral unmixing resulted in at least a doubling of the in vivo sensitivity. Additionally, spectral unmixing also enabled separate imaging of dyes with overlapping spectra which were, without spectral unmixing, not distinguishable.

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