Oleic, Linoleic and Linolenic Acids Increase Ros Production by Fibroblasts Via NADPH Oxidase Activation

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Apr 13

PMID 23579616

Citations 18

Authors

Elaine Hatanaka

Alexandre Dermargos

Aparecida Emiko Hirata

Marco Aurelio Ramirez Vinolo

Angelo Rafael Carpinelli

Philip Newsholme

Hugo Aguirre Armelin

Rui Curi

Affiliations

Soon will be listed here.

Abstract

The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and γ-linolenic acids. ROS production was dependent on the addition of β-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and γ-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47 (phox) phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47 (phox) mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: γ-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and γ-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts.

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