Arabidopsis Phospholipase Dβ1 Modulates Defense Responses to Bacterial and Fungal Pathogens

Overview

Journal New Phytol

Specialty Biology

Date 2013 Apr 13

PMID 23577648

Citations 42

Authors

Jian Zhao

Shivakumar P Devaiah

Cunxi Wang

Maoyin Li

Ruth Welti

Xuemin Wang

Affiliations

Soon will be listed here.

Abstract

Pathogen infection of higher plants often induces rapid production of phosphatidic acid (PA) and changes in lipid profiles, but the enzymatic basis and the function of the lipid change in pathogen-plant interactions are not well understood. Infection of phospholipase D β1 (PLDβ1)-deficient plants by Pseudomonas syringae tomato pv DC3000 (Pst DC30000) resulted in less bacterial growth than in wild-type plants, and the effect was more profound in virulent Pst DC3000 than avirulent Pst DC3000 (carrying the avirulence gene avrRpt2) infection. The expression levels of salicylic acid (SA)-inducible genes were higher, but those inducible by jasmonic acid (JA) showed lower expression in PLDβ1 mutants than in wild-type plants. However, PLDβ1-deficient plants were more susceptible than wild-type plants to the fungus Botrytis cinerea. The PLDβ1-deficient plants had lower levels of PA, JA and JA-related defense gene expression after B. cinerea inoculation. PLDβ1 plays a positive role in pathogen-induced JA production and plant resistance to the necrotrophic fungal pathogen B. cinerea, but a negative role in the SA-dependent signaling pathway and plant tolerance to infection with biotrophic Pst DC3000. PLDβ1 is responsible for most of the increase in PA production in response to necrotrophic B. cinerea and virulent Pst DC3000 infection, but contributes less to avirulent Pst DC3000 (avrRpt2)-induced PA production.

Citing Articles

A GDSL motif-containing lipase modulates Sclerotinia sclerotiorum resistance in Brassica napus.

Ding L, Hu Y, Li T, Li M, Li Y, Wu Y Plant Physiol. 2024; 196(4):2973-2988.

PMID: 39321167 PMC: 11638095. DOI: 10.1093/plphys/kiae500.

Genomic identification and expression profiling of DMP genes in oat (Avena sativa) elucidate their responsiveness to seed aging.

Ma Y, Liu H, Wang J, Zhao G, Niu K, Zhou X BMC Genomics. 2024; 25(1):863.

PMID: 39285326 PMC: 11403964. DOI: 10.1186/s12864-024-10743-y.

Phosphatidic acid signaling and function in nuclei.

Yao S, Kim S, Li J, Tang S, Wang X Prog Lipid Res. 2023; 93:101267.

PMID: 38154743 PMC: 10843600. DOI: 10.1016/j.plipres.2023.101267.

Molecular responses of agroinfiltrated Nicotiana benthamiana leaves expressing suppressor of silencing P19 and influenza virus-like particles.

Hamel L, Tardif R, Poirier-Gravel F, Rasoolizadeh A, Brosseau C, Giroux G Plant Biotechnol J. 2023; 22(5):1078-1100.

PMID: 38041470 PMC: 11022802. DOI: 10.1111/pbi.14247.

Activation of MAPK-mediated immunity by phosphatidic acid in response to positive-strand RNA viruses.

Lin J, Zhao J, Du L, Wang P, Sun B, Zhang C Plant Commun. 2023; 5(1):100659.

PMID: 37434356 PMC: 10811337. DOI: 10.1016/j.xplc.2023.100659.

References

Sang Y, Cui D, Wang X . Phospholipase D and phosphatidic acid-mediated generation of superoxide in Arabidopsis. Plant Physiol. 2001; 126(4):1449-58. PMC: 117145. DOI: 10.1104/pp.126.4.1449. View

Zheng L, Krishnamoorthi R, Zolkiewski M, Wang X . Distinct Ca2+ binding properties of novel C2 domains of plant phospholipase dalpha and beta. J Biol Chem. 2000; 275(26):19700-6. DOI: 10.1074/jbc.M001945200. View

Andersson M, Kourtchenko O, Dangl J, Mackey D, Ellerstrom M . Phospholipase-dependent signalling during the AvrRpm1- and AvrRpt2-induced disease resistance responses in Arabidopsis thaliana. Plant J. 2006; 47(6):947-59. DOI: 10.1111/j.1365-313X.2006.02844.x. View

Welti R, Li W, Li M, Sang Y, Biesiada H, Zhou H . Profiling membrane lipids in plant stress responses. Role of phospholipase D alpha in freezing-induced lipid changes in Arabidopsis. J Biol Chem. 2002; 277(35):31994-2002. DOI: 10.1074/jbc.M205375200. View

Ryu S, Wang X . Activation of phospholipase D and the possible mechanism of activation in wound-induced lipid hydrolysis in castor bean leaves. Biochim Biophys Acta. 1996; 1303(3):243-50. DOI: 10.1016/0005-2760(96)00096-3. View

Zhang Y, Zhu H, Zhang Q, Li M, Yan M, Wang R . Phospholipase dalpha1 and phosphatidic acid regulate NADPH oxidase activity and production of reactive oxygen species in ABA-mediated stomatal closure in Arabidopsis. Plant Cell. 2009; 21(8):2357-77. PMC: 2751945. DOI: 10.1105/tpc.108.062992. View

de Jong C, Laxalt A, Bargmann B, de Wit P, Joosten M, Munnik T . Phosphatidic acid accumulation is an early response in the Cf-4/Avr4 interaction. Plant J. 2004; 39(1):1-12. DOI: 10.1111/j.1365-313X.2004.02110.x. View

Wang X, Devaiah S, Zhang W, Welti R . Signaling functions of phosphatidic acid. Prog Lipid Res. 2006; 45(3):250-78. DOI: 10.1016/j.plipres.2006.01.005. View

Maldonado A, Doerner P, Dixon R, Lamb C, Cameron R . A putative lipid transfer protein involved in systemic resistance signalling in Arabidopsis. Nature. 2002; 419(6905):399-403. DOI: 10.1038/nature00962. View

10.

Rao M, Lee H, Creelman R, Mullet J, Davis K . Jasmonic acid signaling modulates ozone-induced hypersensitive cell death. Plant Cell. 2000; 12(9):1633-46. PMC: 149075. DOI: 10.1105/tpc.12.9.1633. View

11.

de Torres Zabela M, Fernandez-Delmond I, Niittyla T, Sanchez P, Grant M . Differential expression of genes encoding Arabidopsis phospholipases after challenge with virulent or avirulent Pseudomonas isolates. Mol Plant Microbe Interact. 2002; 15(8):808-16. DOI: 10.1094/MPMI.2002.15.8.808. View

12.

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

13.

Yamaguchi T, Kuroda M, Yamakawa H, Ashizawa T, Hirayae K, Kurimoto L . Suppression of a phospholipase D gene, OsPLDbeta1, activates defense responses and increases disease resistance in rice. Plant Physiol. 2009; 150(1):308-19. PMC: 2675732. DOI: 10.1104/pp.108.131979. View

14.

Nandi A, Welti R, Shah J . The Arabidopsis thaliana dihydroxyacetone phosphate reductase gene SUPPRESSSOR OF FATTY ACID DESATURASE DEFICIENCY1 is required for glycerolipid metabolism and for the activation of systemic acquired resistance. Plant Cell. 2004; 16(2):465-77. PMC: 341917. DOI: 10.1105/tpc.016907. View

15.

Yang W, Devaiah S, Pan X, Isaac G, Welti R, Wang X . AtPLAI is an acyl hydrolase involved in basal jasmonic acid production and Arabidopsis resistance to Botrytis cinerea. J Biol Chem. 2007; 282(25):18116-18128. DOI: 10.1074/jbc.M700405200. View

16.

Park J, Gu Y, Lee Y, Yang Z, Lee Y . Phosphatidic acid induces leaf cell death in Arabidopsis by activating the Rho-related small G protein GTPase-mediated pathway of reactive oxygen species generation. Plant Physiol. 2004; 134(1):129-36. PMC: 316293. DOI: 10.1104/pp.103.031393. View

17.

Wang C, Zien C, Afitlhile M, Welti R, Hildebrand D, Wang X . Involvement of phospholipase D in wound-induced accumulation of jasmonic acid in arabidopsis. Plant Cell. 2000; 12(11):2237-46. PMC: 150170. DOI: 10.1105/tpc.12.11.2237. View

18.

Kirik A, Mudgett M . SOBER1 phospholipase activity suppresses phosphatidic acid accumulation and plant immunity in response to bacterial effector AvrBsT. Proc Natl Acad Sci U S A. 2009; 106(48):20532-7. PMC: 2787154. DOI: 10.1073/pnas.0903859106. View

19.

Zhang W, Qin C, Zhao J, Wang X . Phospholipase D alpha 1-derived phosphatidic acid interacts with ABI1 phosphatase 2C and regulates abscisic acid signaling. Proc Natl Acad Sci U S A. 2004; 101(25):9508-13. PMC: 439007. DOI: 10.1073/pnas.0402112101. View

20.

Truman W, Bennett M, Kubigsteltig I, Turnbull C, Grant M . Arabidopsis systemic immunity uses conserved defense signaling pathways and is mediated by jasmonates. Proc Natl Acad Sci U S A. 2007; 104(3):1075-80. PMC: 1783366. DOI: 10.1073/pnas.0605423104. View