Small Molecules Affect Human Dental Pulp Stem Cell Properties Via Multiple Signaling Pathways

Overview

Journal Stem Cells Dev

Date 2013 Apr 12

PMID 23573877

Citations 9

Authors

Mey Al-Habib

Zongdong Yu

George T-J Huang

Affiliations

Soon will be listed here.

Abstract

One fundamental issue regarding stem cells for regenerative medicine is the maintenance of stem cell stemness. The purpose of the study was to test whether small molecules can enhance stem cell properties of mesenchymal stem cells (MSCs) derived from human dental pulp (hDPSCs), which have potential for multiple clinical applications. We identified the effects of small molecules (Pluripotin (SC1), 6-bromoindirubin-3-oxime and rapamycin) on the maintenance of hDPSC properties in vitro and the mechanisms involved in exerting the effects. Primary cultures of hDPSCs were exposed to optimal concentrations of these small molecules. Treated hDPSCs were analyzed for their proliferation, the expression levels of pluripotent and MSC markers, differentiation capacities, and intracellular signaling activations. We found that small molecule treatments decreased cell proliferation and increased the expression of STRO-1, NANOG, OCT4, and SOX2, while diminishing cell differentiation into odonto/osteogenic, adipogenic, and neurogenic lineages in vitro. These effects involved Ras-GAP-, ERK1/2-, and mTOR-signaling pathways, which may preserve the cell self-renewal capacity, while suppressing differentiation. We conclude that small molecules appear to enhance the immature state of hDPSCs in culture, which may be used as a strategy for adult stem cell maintenance and extend their capacity for regenerative applications.

Citing Articles

Research progress on optimization of isolation, cultivation and preservation methods of dental pulp stem cells for clinical application.

Wang X, Li F, Wu S, Xing W, Fu J, Wang R Front Bioeng Biotechnol. 2024; 12:1305614.

PMID: 38633667 PMC: 11021638. DOI: 10.3389/fbioe.2024.1305614.

6-Bromoindirubin-3'-Oxime Regulates Colony Formation, Apoptosis, and Odonto/Osteogenic Differentiation in Human Dental Pulp Stem Cells.

Kornsuthisopon C, Rochanavibhata S, Nowwarote N, Tompkins K, Sukarawan W, Osathanon T Int J Mol Sci. 2022; 23(15).

PMID: 35955809 PMC: 9368902. DOI: 10.3390/ijms23158676.

CGFe and TGF-β1 enhance viability and osteogenic differentiation of human dental pulp stem cells through the MAPK pathway.

Li X, Yang H, Zhang Y, Du X, Yan Z, Li J Exp Ther Med. 2021; 22(4):1048.

PMID: 34434262 PMC: 8353646. DOI: 10.3892/etm.2021.10482.

Potential of tailored amorphous multiporous calcium silicate glass for pulp capping regenerative endodontics-A preliminary assessment.

Liu J, Chen C, Zhu X, Morrow B, Thamma U, Kowal T J Dent. 2021; 109:103655.

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Impact of Sustained Transforming Growth Factor-β Receptor Inhibition on Chromatin Accessibility and Gene Expression in Cultured Human Endometrial MSC.

Lucciola R, Vrljicak P, Gurung S, Filby C, Darzi S, Muter J Front Cell Dev Biol. 2020; 8:567610.

PMID: 32984350 PMC: 7490520. DOI: 10.3389/fcell.2020.567610.

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