Antioxidant Properties and Inhibitory Effects of Satureja Khozestanica Essential Oil on LDL Oxidation Induced-CuSO(4) in Vitro

Overview

Journal Asian Pac J Trop Biomed

Specialty Tropical Medicine

Date 2013 Apr 10

PMID 23570012

Citations 4

Authors

Shahrokh Bagheri

Hassan Ahmadvand

Ali Khosrowbeygi

Farshid Ghazanfari

Narges Jafari

Habibolah Nazem

Reza Haji Hosseini

Affiliations

Soon will be listed here.

Abstract

Objective: To assess various antioxidative activities of Satureja khozestanica essential oil (SKE) and its effect on oxidation of low density lipoprotein (LDL) induced by CuSO4 in vitro by monitoring the formation of conjugated dienes and malondialdehyde (MDA).

Methods: The formation of conjugated dienes, lag time and MDA were measured. Inhibition of this Cu-induced oxidation was studied in the presence of several concentrations of SKE. Also total antioxidant activity and free radical scavenging of SKE were determinated.

Results: It was demonstrated that SKE was able to inhibit LDL oxidation and decrease the resistance of LDL against oxidation. The inhibitory effects of SKE on LDL oxidation were dose-dependent at concentrations ranging from 50 to 200 µg/mL. Total antioxidant capacity of SKE was (3.20±0.40) nmol of ascorbic acid equivalents/g SKE. The SKE showed remarkable scavenging activity on 2, 2-diphenyl-picrylhydrazyl, IC50 (5.30±0.11) ng/mL.

Conclusions: This study shows that SKE is a source of potent antioxidants and prevents the oxidation of LDL in vitro and it may be suitable for use in food and pharmaceutical applications.

Citing Articles

Cytotoxicity, Antiadipogenic, Low-Density Lipoprotein Oxidation Inhibitory Activities, and Acute Toxicity Study of Hydroethanolic Leaf and Bark Extracts.

Mba J, Zouheira D, Guetchueng S, Dairou H, Djouonzo P, Ayong L ScientificWorldJournal. 2024; 2024:1732653.

PMID: 39720344 PMC: 11668551. DOI: 10.1155/tswj/1732653.

Larrea divaricata: anti-inflammatory and antioxidant effects of on macrophages and low density lipoproteins.

Peralta I, Marrassini C, Saint Martin M, Cogoi L, Alonso M, Gugliucci A BMC Complement Med Ther. 2022; 22(1):84.

PMID: 35321702 PMC: 8941816. DOI: 10.1186/s12906-022-03547-8.

Chemical compositions and antifungal activities of against and species.

Motamedi M, Saharkhiz M, Pakshir K, Amini Akbarabadi S, Alikhani Khordshami M, Asadian F Curr Med Mycol. 2020; 5(4):20-25.

PMID: 32104740 PMC: 7034783. DOI: 10.18502/cmm.5.4.2162.

Combined postconditioning with ischemia and cyclosporine-A restore oxidative stress and histopathological changes in reperfusion injury of diabetic myocardium.

Badalzadeh R, Tabatabaei S, Mohammadi M, Khaki A, Mohammadnezhad D Iran J Basic Med Sci. 2017; 20(10):1079-1087.

PMID: 29147482 PMC: 5673691. DOI: 10.22038/IJBMS.2017.9444.

References

Mahboubi M, Kazempour N . Chemical composition and antimicrobial activity of Satureja hortensis and Trachyspermum copticum essential oil. Iran J Microbiol. 2012; 3(4):194-200. PMC: 3330183. View

Morsy M, Mostafa O, Hassan W . A potential protective effect of alpha-tocopherol on vascular complication in spinal cord reperfusion injury in rats. J Biomed Sci. 2010; 17:55. PMC: 2909177. DOI: 10.1186/1423-0127-17-55. View

Castelao J, Gago-Dominguez M . Risk factors for cardiovascular disease in women: relationship to lipid peroxidation and oxidative stress. Med Hypotheses. 2008; 71(1):39-44. DOI: 10.1016/j.mehy.2007.10.016. View

Mosaffa F, Behravan J, Karimi G, Iranshahi M . Antigenotoxic effects of Satureja hortensis L. on rat lymphocytes exposed to oxidative stress. Arch Pharm Res. 2006; 29(2):159-64. DOI: 10.1007/BF02974278. View

Soriano J, Lamprecht B . Chronic obstructive pulmonary disease: a worldwide problem. Med Clin North Am. 2012; 96(4):671-80. DOI: 10.1016/j.mcna.2012.02.005. View

Laranjinha J, Almeida L, Madeira V . Reactivity of dietary phenolic acids with peroxyl radicals: antioxidant activity upon low density lipoprotein peroxidation. Biochem Pharmacol. 1994; 48(3):487-94. DOI: 10.1016/0006-2952(94)90278-x. View

Miller Y, Choi S, Fang L, Tsimikas S . Lipoprotein modification and macrophage uptake: role of pathologic cholesterol transport in atherogenesis. Subcell Biochem. 2010; 51:229-51. DOI: 10.1007/978-90-481-8622-8_8. View

Mahfouz M, Zhou Q, Kummerow F . Effect of curcumin on LDL oxidation in vitro, and lipid peroxidation and antioxidant enzymes in cholesterol fed rabbits. Int J Vitam Nutr Res. 2012; 81(6):378-91. DOI: 10.1024/0300-9831/a000084. View

Ahmadvand H, Bagheri S, Khosrobeigi A, Boshtam M, Abdolahpour F . Effects of olive leaves extract on LDL oxidation induced-CuSO(4) in vitro. Pak J Pharm Sci. 2012; 25(3):571-5. View

10.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

11.

Hajhashemi V, Zolfaghari B, Yousefi A . Antinociceptive and anti-inflammatory activities of Satureja hortensis seed essential oil, hydroalcoholic and polyphenolic extracts in animal models. Med Princ Pract. 2011; 21(2):178-82. DOI: 10.1159/000333555. View

12.

Rababah T, Hettiarachchy N, Horax R, Cho M, Davis B, Dickson J . Thiobarbituric acid reactive substances and volatile compounds in chicken breast meat infused with plant extracts and subjected to electron beam irradiation. Poult Sci. 2006; 85(6):1107-13. DOI: 10.1093/ps/85.6.1107. View

13.

Kishore R, Halim A, Nurul Syazana M, Sirajudeen K . Tualang honey has higher phenolic content and greater radical scavenging activity compared with other honey sources. Nutr Res. 2011; 31(4):322-5. DOI: 10.1016/j.nutres.2011.03.001. View

14.

Haeri S, Minaie B, Amin G, Nikfar S, Khorasani R, Esmaily H . Effect of Satureja khuzestanica essential oil on male rat fertility. Fitoterapia. 2006; 77(7-8):495-9. DOI: 10.1016/j.fitote.2006.05.025. View

15.

Abdollahi M, Salehnia A, Mortazavi S, Ebrahimi M, Shafiee A, Fouladian F . Antioxidant, antidiabetic, antihyperlipidemic,reproduction stimulatory properties and safety of essential oil of Satureja Khuzestanica in rat in vivo: a oxicopharmacological study. Med Sci Monit. 2003; 9(9):BR331-5. View

16.

Leopold J, Loscalzo J . Oxidative risk for atherothrombotic cardiovascular disease. Free Radic Biol Med. 2009; 47(12):1673-706. PMC: 2797369. DOI: 10.1016/j.freeradbiomed.2009.09.009. View

17.

Tatiya A, Surana S, Sutar M, Gamit N . Hepatoprotective effect of poly herbal formulation against various hepatotoxic agents in rats. Pharmacognosy Res. 2012; 4(1):50-6. PMC: 3250040. DOI: 10.4103/0974-8490.91040. View

18.

Jana K, Chatterjee K, Ali K, De D, Bera T, Ghosh D . Antihyperglycemic and antioxidative effects of the hydro-methanolic extract of the seeds of Caesalpinia bonduc on streptozotocin-induced diabetes in male albino rats. Pharmacognosy Res. 2012; 4(1):57-62. PMC: 3250041. DOI: 10.4103/0974-8490.91044. View

19.

Jantan I, Saputri F . Benzophenones and xanthones from Garcinia cantleyana var. cantleyana and their inhibitory activities on human low-density lipoprotein oxidation and platelet aggregation. Phytochemistry. 2012; 80:58-63. DOI: 10.1016/j.phytochem.2012.05.003. View

20.

Yekini I, Hammoudi F, Martin-Nizard F, Yous S, Lebegue N, Berthelot P . Antioxidant activity of benzoxazolinonic and benzothiazolinonic derivatives in the LDL oxidation model. Bioorg Med Chem. 2009; 17(22):7823-30. DOI: 10.1016/j.bmc.2009.09.016. View