Aurora A is Involved in Central Spindle Assembly Through Phosphorylation of Ser 19 in P150Glued

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2013 Apr 3

PMID 23547029

Citations 31

Authors

David Reboutier

Marie-Berengere Troadec

Jean-Yves Cremet

Lucie Chauvin

Vincent Guen

Patrick Salaun

Claude Prigent

Affiliations

Soon will be listed here.

Abstract

Knowledge of Aurora A kinase functions is limited to premetaphase events, particularly centrosome maturation, G2/M transition, and mitotic spindle assembly. The involvement of Aurora A in events after metaphase has only been suggested because appropriate experiments are technically difficult. We report here the design of the first human Aurora A kinase (as-AurA) engineered by chemical genetics techniques. This kinase is fully functional biochemically and in cells, and is rapidly and specifically inhibited by the ATP analogue 1-Naphthyl-PP1 (1-Na-PP1). By treating cells exclusively expressing the as-AurA with 1-Na-PP1, we discovered that Aurora A is required for central spindle assembly in anaphase through phosphorylation of Ser 19 of P150Glued. This paper thus describes a new Aurora A function that takes place after the metaphase-to-anaphase transition and a new powerful tool to search for and study new Aurora A functions.

Citing Articles

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