Purification, Crystallization and Preliminary Crystallographic Analysis of a 4-thiouridine Synthetase-RNA Complex

Overview

Journal Acta Crystallogr Sect F Struct Biol Cryst Commun

Date 2013 Apr 3

PMID 23545650

Citations 2

Authors

Peter Thomas Naumann

Charles T Lauhon

Ralf Ficner

Affiliations

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Abstract

The sulfurtransferase 4-thiouridine synthetase (ThiI) is involved in the ATP-dependent modification of U8 in tRNA. ThiI from Thermotoga maritima was cloned, overexpressed and purified. A complex comprising ThiI and a truncated tRNA was prepared and crystallized, and X-ray diffraction data were collected to a resolution of 3.5 Å. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 102.9, b = 112.8, c = 132.8 Å.

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Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification.

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