Ferumoxytol: a New, Clinically Applicable Label for Stem-cell Tracking in Arthritic Joints with MRI

Overview

Journal Nanomedicine (Lond)

Specialty Biotechnology

Date 2013 Mar 29

PMID 23534832

Citations 50

Authors

Aman Khurana

Hossein Nejadnik

Fanny Chapelin

Olga Lenkov

Rakhee Gawande

Sungmin Lee

Sandeep N Gupta

Nooshin Aflakian

Nikita Derugin

Solomon Messing

Guiting Lin

Tom F Lue

Laura Pisani

Heike E Daldrup-Link

Affiliations

Soon will be listed here.

Abstract

Aim: To develop a clinically applicable MRI technique for tracking stem cells in matrix-associated stem-cell implants, using the US FDA-approved iron supplement ferumoxytol.

Materials & Methods: Ferumoxytol-labeling of adipose-derived stem cells (ADSCs) was optimized in vitro. A total of 11 rats with osteochondral defects of both femurs were implanted with ferumoxytol- or ferumoxides-labeled or unlabeled ADSCs, and underwent MRI up to 4 weeks post matrix-associated stem-cell implant. The signal-to-noise ratio of different matrix-associated stem-cell implant was compared with t-tests and correlated with histopathology.

Results: An incubation concentration of 500 µg iron/ml ferumoxytol and 10 µg/ml protamine sulfate led to significant cellular iron uptake, T2 signal effects and unimpaired ADSC viability. In vivo, ferumoxytol- and ferumoxides-labeled ADSCs demonstrated significantly lower signal-to-noise ratio values compared with unlabeled controls (p < 0.01). Histopathology confirmed engraftment of labeled ADSCs, with slow dilution of the iron label over time.

Conclusion: Ferumoxytol can be used for in vivo tracking of stem cells with MRI.

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