Current Status and Advances in Quantitative Proteomic Mass Spectrometry

Overview

Journal Int J Proteomics

Date 2013 Mar 28

PMID 23533757

Citations 51

Authors

Valerie C Wasinger

Ming Zeng

Yunki Yau

Affiliations

Soon will be listed here.

Abstract

The accurate quantitation of proteins and peptides in complex biological systems is one of the most challenging areas of proteomics. Mass spectrometry-based approaches have forged significant in-roads allowing accurate and sensitive quantitation and the ability to multiplex vastly complex samples through the application of robust bioinformatic tools. These relative and absolute quantitative measures using label-free, tags, or stable isotope labelling have their own strengths and limitations. The continuous development of these methods is vital for increasing reproducibility in the rapidly expanding application of quantitative proteomics in biomarker discovery and validation. This paper provides a critical overview of the primary mass spectrometry-based quantitative approaches and the current status of quantitative proteomics in biomedical research.

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