Anacardic Acids from Cashew Nuts Ameliorate Lung Damage Induced by Exposure to Diesel Exhaust Particles in Mice

Anacardic acids from cashew nut shell liquid, a Brazilian natural substance, have antimicrobial and antioxidant activities and modulate immune responses and angiogenesis. As inflammatory lung diseases have been correlated to environmental pollutants exposure and no reports addressing the effects of dietary supplementation with anacardic acids on lung inflammation in vivo have been evidenced, we investigated the effects of supplementation with anacardic acids in a model of diesel exhaust particle- (DEP-) induced lung inflammation. BALB/c mice received an intranasal instillation of 50 μ g of DEP for 20 days. Ten days prior to DEP instillation, animals were pretreated orally with 50, 150, or 250 mg/kg of anacardic acids or vehicle (100 μ L of cashew nut oil) for 30 days. The biomarkers of inflammatory and antioxidant responses in the alveolar parenchyma, bronchoalveolar lavage fluid (BALF), and pulmonary vessels were investigated. All doses of anacardic acids ameliorated antioxidant enzyme activities and decreased vascular adhesion molecule in vessels. Animals that received 50 mg/kg of anacardic acids showed decreased levels of neutrophils and tumor necrosis factor in the lungs and BALF, respectively. In summary, we demonstrated that AAs supplementation has a potential protective role on oxidative and inflammatory mechanisms in the lungs.

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References

Mudway I, Stenfors N, Duggan S, Roxborough H, Zielinski H, Marklund S . An in vitro and in vivo investigation of the effects of diesel exhaust on human airway lining fluid antioxidants. Arch Biochem Biophys. 2004; 423(1):200-12. DOI: 10.1016/j.abb.2003.12.018. View

Kelly F . Vitamins and respiratory disease: antioxidant micronutrients in pulmonary health and disease. Proc Nutr Soc. 2005; 64(4):510-26. DOI: 10.1079/pns2005457. View

Ramprasath V, Shanthi P, Sachdanandam P . Anti-inflammatory effect of Semecarpus anacardium Linn. Nut extract in acute and chronic inflammatory conditions. Biol Pharm Bull. 2004; 27(12):2028-31. DOI: 10.1248/bpb.27.2028. View

Ballatori N, Krance S, Notenboom S, Shi S, Tieu K, Hammond C . Glutathione dysregulation and the etiology and progression of human diseases. Biol Chem. 2009; 390(3):191-214. PMC: 2756154. DOI: 10.1515/BC.2009.033. View

Al-Humadi N, Siegel P, Lewis D, Barger M, Ma J, Weissman D . Alteration of intracellular cysteine and glutathione levels in alveolar macrophages and lymphocytes by diesel exhaust particle exposure. Environ Health Perspect. 2002; 110(4):349-53. PMC: 1240797. DOI: 10.1289/ehp.02110349. View

Riedl M, Diaz-Sanchez D . Biology of diesel exhaust effects on respiratory function. J Allergy Clin Immunol. 2005; 115(2):221-8. DOI: 10.1016/j.jaci.2004.11.047. View

Haddad J, Harb H . L-gamma-Glutamyl-L-cysteinyl-glycine (glutathione; GSH) and GSH-related enzymes in the regulation of pro- and anti-inflammatory cytokines: a signaling transcriptional scenario for redox(y) immunologic sensor(s)?. Mol Immunol. 2005; 42(9):987-1014. DOI: 10.1016/j.molimm.2004.09.029. View

Kubo J, Lee J, Kubo I . Anti-Helicobacter pylori agents from the cashew apple. J Agric Food Chem. 1999; 47(2):533-7. DOI: 10.1021/jf9808980. View

Paramashivappa R, Phani Kumar P, Subba Rao P, Srinivasa Rao A . Design, synthesis and biological evaluation of benzimidazole/benzothiazole and benzoxazole derivatives as cyclooxygenase inhibitors. Bioorg Med Chem Lett. 2003; 13(4):657-60. DOI: 10.1016/s0960-894x(02)01006-5. View

10.

Flohe L, Gunzler W . Assays of glutathione peroxidase. Methods Enzymol. 1984; 105:114-21. DOI: 10.1016/s0076-6879(84)05015-1. View

11.

Lancas T, Kasahara D, Prado C, Tiberio I, Martins M, Dolhnikoff M . Comparison of early and late responses to antigen of sensitized guinea pig parenchymal lung strips. J Appl Physiol (1985). 2006; 100(5):1610-6. DOI: 10.1152/japplphysiol.00828.2005. View

12.

Sung B, Pandey M, Ahn K, Yi T, Chaturvedi M, Liu M . Anacardic acid (6-nonadecyl salicylic acid), an inhibitor of histone acetyltransferase, suppresses expression of nuclear factor-kappaB-regulated gene products involved in cell survival, proliferation, invasion, and inflammation through inhibition of.... Blood. 2008; 111(10):4880-91. PMC: 2384122. DOI: 10.1182/blood-2007-10-117994. View

13.

DeLeve L, Kaplowitz N . Importance and regulation of hepatic glutathione. Semin Liver Dis. 1990; 10(4):251-66. DOI: 10.1055/s-2008-1040481. View

14.

Vijayalakshmi T, Muthulakshmi V, Sachdanandam P . Effect of the milk extract of Semecarpus anacardium nut on adjuvant arthritis--a dose-dependent study in Wistar albino rats. Gen Pharmacol. 1996; 27(7):1223-6. DOI: 10.1016/s0306-3623(96)00042-0. View

15.

Stenfors N, Nordenhall C, Salvi S, Mudway I, Soderberg M, Blomberg A . Different airway inflammatory responses in asthmatic and healthy humans exposed to diesel. Eur Respir J. 2004; 23(1):82-6. DOI: 10.1183/09031936.03.00004603. View

16.

Dolhnikoff M, da Silva L, de Araujo B, Gomes H, Fernezlian S, Mulder A . The outer wall of small airways is a major site of remodeling in fatal asthma. J Allergy Clin Immunol. 2009; 123(5):1090-7, 1097.e1. DOI: 10.1016/j.jaci.2009.02.032. View

17.

Laks D, de Oliveira R, Afonso de Andre P, Macchione M, Lemos M, Faffe D . Composition of diesel particles influences acute pulmonary toxicity: an experimental study in mice. Inhal Toxicol. 2008; 20(11):1037-42. DOI: 10.1080/08958370802112922. View

18.

Paramashivappa R, Kumar P, Vithayathil P, Rao A . Novel method for isolation of major phenolic constituents from cashew (Anacardium occidentale L.) nut shell liquid. J Agric Food Chem. 2001; 49(5):2548-51. DOI: 10.1021/jf001222j. View

19.

Yoshizaki K, Brito J, Toledo A, Nakagawa N, Piccin V, Junqueira M . Subchronic effects of nasally instilled diesel exhaust particulates on the nasal and airway epithelia in mice. Inhal Toxicol. 2010; 22(7):610-7. DOI: 10.3109/08958371003621633. View

20.

Elsayed N . Antioxidant mobilization in response to oxidative stress: a dynamic environmental-nutritional interaction. Nutrition. 2001; 17(10):828-34. DOI: 10.1016/s0899-9007(01)00646-3. View