Apoptosis Through Bcl-2/Bax and Cleaved Caspase Up-regulation in Melanoma Treated by Boron Neutron Capture Therapy

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Mar 26

PMID 23527236

Citations 9

Authors

Fernanda Faiao-Flores

Paulo Rogerio Pinto Coelho

Joao Dias Toledo Arruda-Neto

Silvya Stuchi Maria-Engler

Manoela Tiago

Vera Luiza Capelozzi

Ricardo Rodrigues Giorgi

Durvanei Augusto Maria

Affiliations

Soon will be listed here.

Abstract

Boron neutron capture therapy (BNCT) is a binary treatment involving selective accumulation of boron carriers in a tumor followed by irradiation with a thermal or epithermal neutron beam. The neutron capture reaction with a boron-10 nucleus yields high linear energy transfer (LET) particles, alpha and (7)Li, with a range of 5 to 9 µm. These particles can only travel very short distances and release their damaging energy directly into the cells containing the boron compound. We aimed to evaluate proliferation, apoptosis and extracellular matrix (ECM) modifications of B16F10 melanoma and normal human melanocytes after BNCT. The amounts of soluble collagen and Hsp47, indicating collagen synthesis in the ECM, as well as the cellular markers of apoptosis, were investigated. BNCT decreased proliferation, altered the ECM by decreasing collagen synthesis and induced apoptosis by regulating Bcl-2/Bax in melanoma. Additionally, BNCT also increased the levels of TNF receptor and the cleaved caspases 3, 7, 8 and 9 in melanoma. These results suggest that multiple pathways related to cell death and cell cycle arrest are involved in the treatment of melanoma by BNCT.

Citing Articles

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