RSV Fusion: Time for a New Model

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2013 Mar 23

PMID 23518574

Citations 22

Authors

Peter Mastrangelo

Richard G Hegele

Affiliations

Soon will be listed here.

Abstract

In this review we propose a partially hypothetical model of respiratory syncytial virus (RSV) binding and entry to the cell that includes the recently discovered RSV receptor nucleolin, in an attempt to stimulate further inquiry in this research area. RSV binding and entry is likely to be a two-step process, the first involving the attachment of the virus to the cell membrane, which may be enhanced by electrostatic interactions with cellular glycoproteins/heparin and the viral G protein, and the second involving fusion to the cell membrane mediated by the viral F protein and a specific cellular fusion receptor. With our recent discovery of nucleolin as a functional fusion receptor for RSV, comes the possibility of a number of new approaches to the development of novel strategies for RSV prophylaxis and therapy, as well as raising some new questions concerning the pathobiology of RSV infection and tropism.

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References

Bugler B, Caizergues-Ferrer M, Bouche G, Bourbon H, Amalric F . Detection and localization of a class of proteins immunologically related to a 100-kDa nucleolar protein. Eur J Biochem. 1982; 128(2-3):475-80. DOI: 10.1111/j.1432-1033.1982.tb06989.x. View

Chen X, Kube D, Cooper M, Davis P . Cell surface nucleolin serves as receptor for DNA nanoparticles composed of pegylated polylysine and DNA. Mol Ther. 2007; 16(2):333-42. DOI: 10.1038/sj.mt.6300365. View

BLOUNT Jr R, Morris J, Savage R . Recovery of cytopathogenic agent from chimpanzees with coryza. Proc Soc Exp Biol Med. 1956; 92(3):544-9. DOI: 10.3181/00379727-92-22538. View

Harcourt J, Alvarez R, Jones L, Henderson C, Anderson L, Tripp R . Respiratory syncytial virus G protein and G protein CX3C motif adversely affect CX3CR1+ T cell responses. J Immunol. 2006; 176(3):1600-8. DOI: 10.4049/jimmunol.176.3.1600. View

Schwab M, Dreyer C . Protein phosphorylation sites regulate the function of the bipartite NLS of nucleolin. Eur J Cell Biol. 1997; 73(4):287-97. View

Chen C, Chiang S, Yeh N . Increased stability of nucleolin in proliferating cells by inhibition of its self-cleaving activity. J Biol Chem. 1991; 266(12):7754-8. View

Srinivasakumar N, Ogra P, Flanagan T . Characteristics of fusion of respiratory syncytial virus with HEp-2 cells as measured by R18 fluorescence dequenching assay. J Virol. 1991; 65(8):4063-9. PMC: 248838. DOI: 10.1128/JVI.65.8.4063-4069.1991. View

Barel M, Hovanessian A, Meibom K, Briand J, Dupuis M, Charbit A . A novel receptor - ligand pathway for entry of Francisella tularensis in monocyte-like THP-1 cells: interaction between surface nucleolin and bacterial elongation factor Tu. BMC Microbiol. 2008; 8:145. PMC: 2551611. DOI: 10.1186/1471-2180-8-145. View

Tajrishi M, Tuteja R, Tuteja N . Nucleolin: The most abundant multifunctional phosphoprotein of nucleolus. Commun Integr Biol. 2011; 4(3):267-75. PMC: 3187884. DOI: 10.4161/cib.4.3.14884. View

10.

Song N, Ding Y, Zhuo W, He T, Fu Z, Chen Y . The nuclear translocation of endostatin is mediated by its receptor nucleolin in endothelial cells. Angiogenesis. 2012; 15(4):697-711. DOI: 10.1007/s10456-012-9284-y. View

11.

Reyes-Reyes E, Akiyama S . Cell-surface nucleolin is a signal transducing P-selectin binding protein for human colon carcinoma cells. Exp Cell Res. 2008; 314(11-12):2212-23. PMC: 2504360. DOI: 10.1016/j.yexcr.2008.03.016. View

12.

Chang T, Segovia J, Sabbah A, Mgbemena V, Bose S . Cholesterol-rich lipid rafts are required for release of infectious human respiratory syncytial virus particles. Virology. 2011; 422(2):205-13. PMC: 3249476. DOI: 10.1016/j.virol.2011.10.029. View

13.

Techaarpornkul S, Barretto N, Peeples M . Functional analysis of recombinant respiratory syncytial virus deletion mutants lacking the small hydrophobic and/or attachment glycoprotein gene. J Virol. 2001; 75(15):6825-34. PMC: 114409. DOI: 10.1128/JVI.75.15.6825-6834.2001. View

14.

Malhotra R, Ward M, Bright H, Priest R, Foster M, Hurle M . Isolation and characterisation of potential respiratory syncytial virus receptor(s) on epithelial cells. Microbes Infect. 2003; 5(2):123-33. DOI: 10.1016/s1286-4579(02)00079-5. View

15.

Nisole S, Krust B, Hovanessian A . Anchorage of HIV on permissive cells leads to coaggregation of viral particles with surface nucleolin at membrane raft microdomains. Exp Cell Res. 2002; 276(2):155-73. DOI: 10.1006/excr.2002.5522. View

16.

McLellan J, Yang Y, Graham B, Kwong P . Structure of respiratory syncytial virus fusion glycoprotein in the postfusion conformation reveals preservation of neutralizing epitopes. J Virol. 2011; 85(15):7788-96. PMC: 3147929. DOI: 10.1128/JVI.00555-11. View

17.

Losfeld M, El Khoury D, Mariot P, Carpentier M, Krust B, Briand J . The cell surface expressed nucleolin is a glycoprotein that triggers calcium entry into mammalian cells. Exp Cell Res. 2008; 315(2):357-69. DOI: 10.1016/j.yexcr.2008.10.039. View

18.

Greco A, Arata L, Soler E, Gaume X, Coute Y, Hacot S . Nucleolin interacts with US11 protein of herpes simplex virus 1 and is involved in its trafficking. J Virol. 2011; 86(3):1449-57. PMC: 3264372. DOI: 10.1128/JVI.06194-11. View

19.

Kahn J, Schnell M, Buonocore L, Rose J . Recombinant vesicular stomatitis virus expressing respiratory syncytial virus (RSV) glycoproteins: RSV fusion protein can mediate infection and cell fusion. Virology. 1999; 254(1):81-91. DOI: 10.1006/viro.1998.9535. View

20.

Taylor G, Stott E, Furze J, Ford J, Sopp P . Protective epitopes on the fusion protein of respiratory syncytial virus recognized by murine and bovine monoclonal antibodies. J Gen Virol. 1992; 73 ( Pt 9):2217-23. DOI: 10.1099/0022-1317-73-9-2217. View