BMSCs Reduce Rat Granulosa Cell Apoptosis Induced by Cisplatin and Perimenopause

Overview

Journal BMC Cell Biol

Publisher Biomed Central

Specialty Cell Biology

Date 2013 Mar 21

PMID 23510080

Citations 37

Authors

Jun-Qi Guo

Xia Gao

Zhi-Jie Lin

Wei-Zhen Wu

Liang-Hu Huang

Hui-Yue Dong

Jin Chen

Jun Lu

Yun-Fen Fu

Jin Wang

Yu-Jie Ma

Xiao-Wen Chen

Zhi-Xian Wu

Fu-Qiang He

Shun-Liang Yang

Lian-Ming Liao

Feng Zheng

Jian-Ming Tan

Affiliations

Soon will be listed here.

Abstract

Background: The objective of this study was to evaluate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of granulosa cells (GCs) in rats.

Results: Cisplatin increased GC apoptosis from 0.59% to 13.04% in the control and cisplatin treatment groups, respectively, which was significantly reduced upon co-culture with BMSCs to 4.84%. Cisplatin treatment increased p21 and bax and decreased c-myc mRNA expression, which was reversed upon co-culture with BMSCs. As compared to young rats, increased apoptosis was observed in the perimenopausal rats (P < 0.001). After 3 months, the apoptosis rate in the BMSC group was significantly lower than that of the control group (P = 0.007).

Conclusions: BMSC therapy may protect against GC apoptosis induced by cisplatin and perimenopause. Further studies are necessary to evaluate therapeutic efficacy of BMSCs.

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