Cysteine Protease-binding Protein Family 6 Mediates the Trafficking of Amylases to Phagosomes in the Enteric Protozoan Entamoeba Histolytica

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2013 Mar 20

PMID 23509141

Citations 15

Authors

Atsushi Furukawa

Kumiko Nakada-Tsukui

Tomoyoshi Nozaki

Affiliations

Soon will be listed here.

Abstract

Phagocytosis plays a pivotal role in nutrient acquisition and evasion from the host defense systems in Entamoeba histolytica, the intestinal protozoan parasite that causes amoebiasis. We previously reported that E. histolytica possesses a unique class of a hydrolase receptor family, designated the cysteine protease-binding protein family (CPBF), that is involved in trafficking of hydrolases to lysosomes and phagosomes, and we have also reported that CPBF1 and CPBF8 bind to cysteine proteases or β-hexosaminidase α-subunit and lysozymes, respectively. In this study, we showed by immunoprecipitation that CPBF6, one of the most highly expressed CPBF proteins, specifically binds to α-amylase and γ-amylase. We also found that CPBF6 is localized in lysosomes, based on immunofluorescence imaging. Immunoblot and proteome analyses of the isolated phagosomes showed that CPBF6 mediates transport of amylases to phagosomes. We also demonstrated that the carboxyl-terminal cytosolic region of CPBF6 is engaged in the regulation of the trafficking of CPBF6 to phagosomes. Our proteome analysis of phagosomes also revealed new potential phagosomal proteins.

Citing Articles

Molecular Dissection of Phagocytosis by Proteomic Analysis in .

Watanabe N, Nakada-Tsukui K, Nozaki T Genes (Basel). 2023; 14(2).

PMID: 36833306 PMC: 9957367. DOI: 10.3390/genes14020379.

Proteomic analysis of Atg8-dependent recruitment of phagosomal proteins in the enteric protozoan parasite .

Nakada-Tsukui K, Watanabe N, Shibata K, Wahyuni R, Miyamoto E, Nozaki T Front Cell Infect Microbiol. 2022; 12:961645.

PMID: 36262186 PMC: 9575557. DOI: 10.3389/fcimb.2022.961645.

Trogocytosis in Unicellular Eukaryotes.

Nakada-Tsukui K, Nozaki T Cells. 2021; 10(11).

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Unraveling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica through proteomic analysis.

Salgado-Martinez A, Avila-Bonilla R, Ramirez-Moreno E, Castanon-Sanchez C, Lopez-Camarillo C, Marchat L FEBS Open Bio. 2021; 11(10):2819-2835.

PMID: 34486252 PMC: 8487052. DOI: 10.1002/2211-5463.13287.

Two isotypes of phosphatidylinositol 3-phosphate-binding sorting nexins play distinct roles in trogocytosis in Entamoeba histolytica.

Watanabe N, Nakada-Tsukui K, Nozaki T Cell Microbiol. 2019; 22(3):e13144.

PMID: 31713312 PMC: 7027479. DOI: 10.1111/cmi.13144.

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