Correlation of Expression and Activity of Matrix Metalloproteinase-9 and -2 in Human Gingival Cells of Periodontitis Patients

Overview

Journal J Periodontal Implant Sci

Specialty Dentistry

Date 2013 Mar 20

PMID 23507779

Citations 9

Authors

Kyung-A Kim

Soo-Bong Chung

Eun-Young Hawng

Seung-Hyun Noh

Kwon-Ho Song

Hanna-Hyun Kim

Cheorl-Ho Kim

Young-Guk Park

Affiliations

Soon will be listed here.

Abstract

Purpose: Matrix metalloproteinases (MMPs) are capable of degrading extracellular matrix, and they are inducible enzymes depending on an inflammatory environment such as periodontitis and bacterial infection in periodontal tissue. Gingival inflammation has been postulated to be correlated with the production of MMP-2 and MMP-9. The objective of this study was to quantify the expression and activity of MMP-9 and -2, and to determine the correlation between activity and expression of these MMPs in human gingival tissues with periodontitis.

Methods: The gingival tissues of 13 patients were homogenized in 500 µL of phosphate buffered saline with a protease inhibitor cocktail. The expression and activity of MMP-2 and -9 were measured by enzyme-linked immunosorbent assay and Western blot analysis, and quantified by a densitometer. For the correlation line, statistical analysis was performed using the Systat software package.

Results: MMP-9 was highly expressed in all gingival tissue samples, whereas MMP-2 was underexpressed compared with MMP-9. MMP-9 activity increased together with the MMP-9 expression level, with a positive correlation (r=0.793, P=0.01). The correlation was not observed in MMP-2.

Conclusions: The expression of MMP-2 and -9 might contribute to periodontal physiological and pathological processes, and the degree of MMP-9 expression and activity are predictive indicators relevant to the progression of periodontitis.

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References

Rai B, Kharb S, Jain R, Anand S . Biomarkers of periodontitis in oral fluids. J Oral Sci. 2008; 50(1):53-6. DOI: 10.2334/josnusd.50.53. View

Nishikawa M, Yamaguchi Y, Yoshitake K, Saeki Y . Effects of TNFalpha and prostaglandin E2 on the expression of MMPs in human periodontal ligament fibroblasts. J Periodontal Res. 2002; 37(3):167-76. DOI: 10.1034/j.1600-0765.2002.00656.x. View

Cox S, Eley B, Kiili M, Asikainen A, Tervahartiala T, Sorsa T . Collagen degradation by interleukin-1beta-stimulated gingival fibroblasts is accompanied by release and activation of multiple matrix metalloproteinases and cysteine proteinases. Oral Dis. 2006; 12(1):34-40. DOI: 10.1111/j.1601-0825.2005.01153.x. View

Kesanakurti D, Chetty C, Bhoopathi P, Lakka S, Gorantla B, Tsung A . Suppression of MMP-2 attenuates TNF-α induced NF-κB activation and leads to JNK mediated cell death in glioma. PLoS One. 2011; 6(5):e19341. PMC: 3087754. DOI: 10.1371/journal.pone.0019341. View

Marton I, Kiss C . Protective and destructive immune reactions in apical periodontitis. Oral Microbiol Immunol. 2001; 15(3):139-50. DOI: 10.1034/j.1399-302x.2000.150301.x. View

Reynolds J, Meikle M . Mechanisms of connective tissue matrix destruction in periodontitis. Periodontol 2000. 1997; 14:144-57. DOI: 10.1111/j.1600-0757.1997.tb00195.x. View

DeCarlo Jr A, Windsor L, Bodden M, Harber G, Birkedal-Hansen H . Activation and novel processing of matrix metalloproteinases by a thiol-proteinase from the oral anaerobe Porphyromonas gingivalis. J Dent Res. 1997; 76(6):1260-70. DOI: 10.1177/00220345970760060501. View

Van Dyke T, Serhan C . Resolution of inflammation: a new paradigm for the pathogenesis of periodontal diseases. J Dent Res. 2003; 82(2):82-90. DOI: 10.1177/154405910308200202. View

Birkedal-Hansen H . Role of cytokines and inflammatory mediators in tissue destruction. J Periodontal Res. 1993; 28(6 Pt 2):500-10. DOI: 10.1111/j.1600-0765.1993.tb02113.x. View

10.

Vernal R, Dezerega A, Dutzan N, Chaparro A, Leon R, Chandia S . RANKL in human periapical granuloma: possible involvement in periapical bone destruction. Oral Dis. 2006; 12(3):283-9. DOI: 10.1111/j.1601-0825.2005.01191.x. View

11.

Sorsa T, Tjaderhane L, Salo T . Matrix metalloproteinases (MMPs) in oral diseases. Oral Dis. 2004; 10(6):311-8. DOI: 10.1111/j.1601-0825.2004.01038.x. View

12.

Ingman T, Sorsa T, Michaelis J, Konttinen Y . Matrix metalloproteinases-1, -3, and -8 in adult periodontitis in situ. An immunohistochemical study. Ann N Y Acad Sci. 1994; 732:459-61. DOI: 10.1111/j.1749-6632.1994.tb24785.x. View

13.

Emingil G, Kuula H, Sorsa T, Atilla G . Gingival crevicular fluid matrix metalloproteinase-25 and -26 levels in periodontal disease. J Periodontol. 2006; 77(4):664-71. DOI: 10.1902/jop.2006.050288. View

14.

Tervahartiala T, Pirila E, Ceponis A, Maisi P, Salo T, Tuter G . The in vivo expression of the collagenolytic matrix metalloproteinases (MMP-2, -8, -13, and -14) and matrilysin (MMP-7) in adult and localized juvenile periodontitis. J Dent Res. 2001; 79(12):1969-77. DOI: 10.1177/00220345000790120801. View

15.

Murphy G, Docherty A, Hembry R, Reynolds J . Metalloproteinases and tissue damage. Br J Rheumatol. 1991; 30 Suppl 1:25-31. View

16.

Sorsa T, Tervahartiala T, Leppilahti J, Hernandez M, Gamonal J, Tuomainen A . Collagenase-2 (MMP-8) as a point-of-care biomarker in periodontitis and cardiovascular diseases. Therapeutic response to non-antimicrobial properties of tetracyclines. Pharmacol Res. 2010; 63(2):108-13. DOI: 10.1016/j.phrs.2010.10.005. View

17.

Uitto V, Overall C, McCulloch C . Proteolytic host cell enzymes in gingival crevice fluid. Periodontol 2000. 2003; 31:77-104. DOI: 10.1034/j.1600-0757.2003.03106.x. View

18.

Aiba T, Akeno N, Kawane T, Okamoto H, Horiuchi N . Matrix metalloproteinases-1 and -8 and TIMP-1 mRNA levels in normal and diseased human gingivae. Eur J Oral Sci. 1996; 104(5-6):562-9. DOI: 10.1111/j.1600-0722.1996.tb00142.x. View

19.

Tsagareli Z, Shishniashvili T, Gogiashvili L, Kvachadze T, Khimshiashvili N . [The level of matrix metalloproteinases and type IV collagen in the gingival mucosa under different clinical forms of periodontitis in pre-and pubertal periods and their prognostic value]. Georgian Med News. 2012; (206):25-9. View

20.

Corotti M, Zambuzzi W, Paiva K, Menezes R, Pinto L, Lara V . Immunolocalization of matrix metalloproteinases-2 and -9 during apical periodontitis development. Arch Oral Biol. 2009; 54(8):764-71. DOI: 10.1016/j.archoralbio.2009.04.013. View