Effect of Linseed Oil Dietary Supplementation on Fatty Acid Composition and Gene Expression in Adipose Tissue of Growing Goats

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2013 Mar 14

PMID 23484090

Citations 18

Authors

M Ebrahimi

M A Rajion

Y M Goh

A Q Sazili

J T Schonewille

Affiliations

Soon will be listed here.

Abstract

This study was conducted to determine the effects of feeding oil palm frond silage based diets with added linseed oil (LO) containing high α -linolenic acid (C18:3n-3), namely, high LO (HLO), low LO (LLO), and without LO as the control group (CON) on the fatty acid (FA) composition of subcutaneous adipose tissue and the gene expression of peroxisome proliferator-activated receptor (PPAR) α , PPAR- γ , and stearoyl-CoA desaturase (SCD) in Boer goats. The proportion of C18:3n-3 in subcutaneous adipose tissue was increased (P < 0.01) by increasing the LO in the diet, suggesting that the FA from HLO might have escaped ruminal biohydrogenation. Animals fed HLO diets had lower proportions of C18:1 trans-11, C18:2n-6, CLA cis-9 trans-11, and C20:4n-6 and higher proportions of C18:3n-3, C22:5n-3, and C22:6n-3 in the subcutaneous adipose tissue than animals fed the CON diets, resulting in a decreased n-6:n-3 fatty acid ratio (FAR) in the tissue. In addition, feeding the HLO diet upregulated the expression of PPAR- γ (P < 0.05) but downregulated the expression of SCD (P < 0.05) in the adipose tissue. The results of the present study show that LO can be safely incorporated in the diets of goats to enrich goat meat with potential health beneficial FA (i.e., n-3 FA).

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References

Bellinger L, Lilley C, Langley-Evans S . Prenatal exposure to a maternal low-protein diet programmes a preference for high-fat foods in the young adult rat. Br J Nutr. 2004; 92(3):513-20. DOI: 10.1079/bjn20041224. View

Daniel Z, Wynn R, Salter A, Buttery P . Differing effects of forage and concentrate diets on the oleic acid and conjugated linoleic acid content of sheep tissues: the role of stearoyl-CoA desaturase. J Anim Sci. 2004; 82(3):747-58. DOI: 10.2527/2004.823747x. View

De La Torre A, Gruffat D, Durand D, Micol D, Peyron A, Scislowski V . Factors influencing proportion and composition of CLA in beef. Meat Sci. 2011; 73(2):258-68. DOI: 10.1016/j.meatsci.2005.11.025. View

Van Soest P, Robertson J, Lewis B . Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci. 1991; 74(10):3583-97. DOI: 10.3168/jds.S0022-0302(91)78551-2. View

Pawar A, Jump D . Unsaturated fatty acid regulation of peroxisome proliferator-activated receptor alpha activity in rat primary hepatocytes. J Biol Chem. 2003; 278(38):35931-9. DOI: 10.1074/jbc.M306238200. View

Tontonoz P, Spiegelman B . Fat and beyond: the diverse biology of PPARgamma. Annu Rev Biochem. 2008; 77:289-312. DOI: 10.1146/annurev.biochem.77.061307.091829. View

Graugnard D, Piantoni P, Bionaz M, Berger L, Faulkner D, Loor J . Adipogenic and energy metabolism gene networks in longissimus lumborum during rapid post-weaning growth in Angus and Angus x Simmental cattle fed high-starch or low-starch diets. BMC Genomics. 2009; 10:142. PMC: 2676302. DOI: 10.1186/1471-2164-10-142. View

Wahle K, Heys S, Rotondo D . Conjugated linoleic acids: are they beneficial or detrimental to health?. Prog Lipid Res. 2004; 43(6):553-87. DOI: 10.1016/j.plipres.2004.08.002. View

Dervishi E, Serrano C, Joy M, Serrano M, Rodellar C, Calvo J . The effect of feeding system in the expression of genes related with fat metabolism in semitendinous muscle in sheep. Meat Sci. 2011; 89(1):91-7. DOI: 10.1016/j.meatsci.2011.04.003. View

10.

Wachira A, Sinclair L, Wilkinson R, Enser M, Wood J, Fisher A . Effects of dietary fat source and breed on the carcass composition, n-3 polyunsaturated fatty acid and conjugated linoleic acid content of sheep meat and adipose tissue. Br J Nutr. 2002; 88(6):697-709. DOI: 10.1079/BJN2002727. View

11.

Givens D . The role of animal nutrition in improving the nutritive value of animal-derived foods in relation to chronic disease. Proc Nutr Soc. 2005; 64(3):395-402. DOI: 10.1079/pns2005448. View

12.

Al-Hasani H, Joost H . Nutrition-/diet-induced changes in gene expression in white adipose tissue. Best Pract Res Clin Endocrinol Metab. 2005; 19(4):589-603. DOI: 10.1016/j.beem.2005.07.005. View

13.

Moloney A, Kennedy C, Noci F, Monahan F, Kerry J . Lipid and colour stability of M. longissimus muscle from lambs fed camelina or linseed as oil or seeds. Meat Sci. 2012; 92(1):1-7. DOI: 10.1016/j.meatsci.2012.03.011. View

14.

Ntambi J . Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. J Lipid Res. 1999; 40(9):1549-58. View

15.

Ntambi J, Miyazaki M . Regulation of stearoyl-CoA desaturases and role in metabolism. Prog Lipid Res. 2003; 43(2):91-104. DOI: 10.1016/s0163-7827(03)00039-0. View

16.

Harfoot C, Noble R, Moore J . Factors influencing the extent of biohydrogenation of linoleic acid by rumen micro-organisms in vitro. J Sci Food Agric. 1973; 24(8):961-70. DOI: 10.1002/jsfa.2740240814. View

17.

Simopoulos A . Evolutionary aspects of diet, the omega-6/omega-3 ratio and genetic variation: nutritional implications for chronic diseases. Biomed Pharmacother. 2006; 60(9):502-7. DOI: 10.1016/j.biopha.2006.07.080. View

18.

Sampath H, Ntambi J . Polyunsaturated fatty acid regulation of genes of lipid metabolism. Annu Rev Nutr. 2005; 25:317-40. DOI: 10.1146/annurev.nutr.25.051804.101917. View

19.

Turpeinen A, Mutanen M, Aro A, Salminen I, Basu S, Palmquist D . Bioconversion of vaccenic acid to conjugated linoleic acid in humans. Am J Clin Nutr. 2002; 76(3):504-10. DOI: 10.1093/ajcn/76.3.504. View

20.

Dewhurst R, Scollan N, Lee M, Ougham H, Humphreys M . Forage breeding and management to increase the beneficial fatty acid content of ruminant products. Proc Nutr Soc. 2003; 62(2):329-36. DOI: 10.1079/pns2003241. View