Gas2l3, a Novel Constriction Site-associated Protein Whose Regulation is Mediated by the APC/C Cdh1 Complex

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Mar 8

PMID 23469016

Citations 16

Authors

Tal Peer

Roxane Lahmi

Yaara Sharaby

Evelin Chorni

Meirav Noach

Manuela Vecsler

Eitan Zlotorynski

Hanno Steen

Judith A Steen

Amit Tzur

Affiliations

Soon will be listed here.

Abstract

Growth arrest-specific 2-like protein 3 (Gas2l3) was recently identified as an Actin/Tubulin cross-linker protein that regulates cytokinesis. Using cell-free systems from both frog eggs and human cells, we show that the Gas2l3 protein is targeted for ubiquitin-mediated proteolysis by the APC/C(Cdh1) complex, but not by the APC/C(Cdc20) complex, and is phosphorylated by Cdk1 in mitosis. Moreover, late in cytokinesis, Gas2l3 is exclusively localized to the constriction sites, which are the narrowest parts of the intercellular bridge connecting the two daughter cells. Overexpression of Gas2l3 specifically interferes with cell abscission, which is the final stage of cell division, when the cutting of the intercellular bridge at the constriction sites occurs. We therefore suggest that Gas2l3 is part of the cellular mechanism that terminates cell division.

Citing Articles

Cytokinetic abscission requires actin-dependent microtubule severing.

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Elevated GAS2L3 Expression Correlates With Poor Prognosis in Patients With Glioma: A Study Based on Bioinformatics and Immunohistochemical Analysis.

Zhou Y, Zhang L, Song S, Xu L, Yan Y, Wu H Front Genet. 2021; 12:649270.

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