Fecal Microbiota Diversity in Survivors of Adolescent/young Adult Hodgkin Lymphoma: a Study of Twins

Overview

Journal Br J Cancer

Specialty Oncology

Date 2013 Feb 28

PMID 23443674

Citations 30

Authors

W Cozen

G Yu

M H Gail

V K Ridaura

B N Nathwani

A E Hwang

A S Hamilton

T M Mack

J I Gordon

J J Goedert

Affiliations

Soon will be listed here.

Abstract

Background: Adolescent/young adult Hodgkin lymphoma (AYAHL) survivors report fewer exposures to infections during childhood compared with controls, and they have functional lymphocyte aberrations. The gut microbiota plays a central role in immunity.

Methods: We investigated whether fecal microbial diversity differed between 13 AYAHL survivors and their unaffected co-twin controls. Pyrosequencing of fecal bacterial 16S rRNA amplicons yielded 252 943 edited reads that were assigned to species-level operational taxonomic units (OTUs) and standardised for sequencing depth by random sampling. Microbial diversity was compared within vs between twin pairs and by case-control status.

Results: The number of unique OTUs was more similar within twin pairs compared with randomly paired participants (P=0.0004). The AYAHL cases had fewer unique OTUs compared with their co-twin controls (338 vs 369, P=0.015); this difference was not significant (169 vs 183, P=0.10) when restricted to abundant OTUs.

Conclusion: In this small study, AYAHL survivors appear to have a deficit of rare gut microbes. Further work is needed to determine if reduced microbial diversity is a consequence of the disease, its treatment, or a particularly hygienic environment.

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