Enhanced Dihydropyridine Receptor Calcium Channel Activity Restores Muscle Strength in JP45/CASQ1 Double Knockout Mice

Overview

Journal Nat Commun

Specialty Biology

Date 2013 Feb 28

PMID 23443569

Citations 25

Authors

Barbara Mosca

Osvaldo Delbono

Maria Laura Messi

Leda Bergamelli

Zhong-Min Wang

Mirko Vukcevic

Ruben Lopez

Susan Treves

Miyuki Nishi

Hiroshi Takeshima

Cecilia Paolini

Marta Martini

Giorgio Rispoli

Feliciano Protasi

Francesco Zorzato

Affiliations

Soon will be listed here.

Abstract

Muscle strength declines with age in part due to a decline of Ca(2+) release from sarcoplasmic reticulum calcium stores. Skeletal muscle dihydropyridine receptors (Ca(v)1.1) initiate muscle contraction by activating ryanodine receptors in the sarcoplasmic reticulum. Ca(v)1.1 channel activity is enhanced by a retrograde stimulatory signal delivered by the ryanodine receptor. JP45 is a membrane protein interacting with Ca(v)1.1 and the sarcoplasmic reticulum Ca(2+) storage protein calsequestrin (CASQ1). Here we show that JP45 and CASQ1 strengthen skeletal muscle contraction by modulating Ca(v)1.1 channel activity. Using muscle fibres from JP45 and CASQ1 double knockout mice, we demonstrate that Ca(2+) transients evoked by tetanic stimulation are the result of massive Ca(2+) influx due to enhanced Ca(v)1.1 channel activity, which restores muscle strength in JP45/CASQ1 double knockout mice. We envision that JP45 and CASQ1 may be candidate targets for the development of new therapeutic strategies against decay of skeletal muscle strength caused by a decrease in sarcoplasmic reticulum Ca(2+) content.

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