Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2013 Feb 28

PMID 23443395

Citations 3

Authors

Wenjing Tian

Heidi Qunhui Xie

Hualing Fu

Xinhui Pei

Bin Zhao

Affiliations

Soon will be listed here.

Abstract

With the development of biotechnology, approaches based on antibodies, such as enzyme-linked immunosorbent assay (ELISA), active aryl hydrocarbon immunoassay (Ah-I) and other multi-analyte immunoassays, have been utilized as alternatives to the conventional techniques based on gas chromatography and mass spectroscopy for the analysis of dioxin and dioxin-like compounds in environmental and biological samples. These screening methods have been verified as rapid, simple and cost-effective. This paper provides an overview on the development and application of antibody-based approaches, such as ELISA, Ah-I, and multi-analyte immunoassays, covering the sample extraction and cleanup, antigen design, antibody preparation and immunoanalysis. However, in order to meet the requirements for on-site fast detection and relative quantification of dioxins in the environment, further optimization is needed to make these immuno-analytical methods more sensitive and easy to use.

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References

Yang J, Lee H, Park K . Development of human dermal epithelial cell-based bioassay for the dioxins. Chemosphere. 2008; 72(8):1188-92. DOI: 10.1016/j.chemosphere.2008.03.012. View

Kaware M, Bronshtein A, Safi J, Van Emon J, Chuang J, Hock B . Enzyme-linked immunosorbent assay (ELISA) and sol-gel-based immunoaffinity purification (IAP) of the pyrethroid bioallethrin in food and environmental samples. J Agric Food Chem. 2006; 54(18):6482-92. DOI: 10.1021/jf0607415. View

Pack P, Pluckthun A . Miniantibodies: use of amphipathic helices to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli. Biochemistry. 1992; 31(6):1579-84. DOI: 10.1021/bi00121a001. View

Behnisch P, Hosoe K, Sakai S . Brominated dioxin-like compounds: in vitro assessment in comparison to classical dioxin-like compounds and other polyaromatic compounds. Environ Int. 2003; 29(6):861-77. DOI: 10.1016/s0160-4120(03)00105-3. View

Behnisch P, Hosoe K, Sakai S . Bioanalytical screening methods for dioxins and dioxin-like compounds a review of bioassay/biomarker technology. Environ Int. 2002; 27(5):413-39. DOI: 10.1016/s0160-4120(01)00028-9. View

Johnson J, Van Emon J . Quantitative enzyme-linked immunosorbent assay for determination of polychlorinated biphenyls in environmental soil and sediment samples. Anal Chem. 2011; 68(1):162-9. DOI: 10.1021/ac950410j. View

Chen H, Zhuang H . Real-time immuno-PCR assay for detecting PCBs in soil samples. Anal Bioanal Chem. 2009; 394(4):1205-11. DOI: 10.1007/s00216-009-2787-8. View

Ohmura N, Tsukidate Y, Shinozaki H, Lackie S, Saiki H . Combinational use of antibody affinities in an immunoassay for extension of dynamic range and detection of multiple analytes. Anal Chem. 2003; 75(1):104-10. DOI: 10.1021/ac020247+. View

Barletta J, Edelman D, Highsmith W, Constantine N . Detection of ultra-low levels of pathologic prion protein in scrapie infected hamster brain homogenates using real-time immuno-PCR. J Virol Methods. 2005; 127(2):154-64. DOI: 10.1016/j.jviromet.2005.04.007. View

10.

Sugawara Y, Saito K, Ogawa M, Kobayashi S, Shan G, Sanborn J . Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay--assay validation for human milk. Chemosphere. 2002; 46(9-10):1471-6. DOI: 10.1016/s0045-6535(01)00267-3. View

11.

Van Emon J, Gerlach C, Bowman K . Bioseparation and bioanalytical techniques in environmental monitoring. J Chromatogr B Biomed Sci Appl. 1998; 715(1):211-28. DOI: 10.1016/s0378-4347(98)00261-8. View

12.

Garrison P, Tullis K, Aarts J, Brouwer A, Giesy J, Denison M . Species-specific recombinant cell lines as bioassay systems for the detection of 2,3,7,8-tetrachlorodibenzo-p-dioxin-like chemicals. Fundam Appl Toxicol. 1996; 30(2):194-203. DOI: 10.1006/faat.1996.0056. View

13.

Hoogenboom R, Bovee T, Traag W, Hoogerbrugge R, Baumann B, Portier L . The use of the DR CALUX bioassay and indicator polychlorinated biphenyls for screening of elevated levels of dioxins and dioxin-like polychlorinated biphenyls in eel. Mol Nutr Food Res. 2006; 50(10):945-57. DOI: 10.1002/mnfr.200600061. View

14.

Bradfield C, Poland A . A competitive binding assay for 2,3,7,8-tetrachlorodibenzo-p-dioxin and related ligands of the Ah receptor. Mol Pharmacol. 1988; 34(5):682-8. View

15.

Glass T, Ohmura N, Taemi Y, Joh T . Simple immunoassay for detection of PCBs in transformer oil. Environ Sci Technol. 2005; 39(13):5005-9. DOI: 10.1021/es048012y. View

16.

Albro P, Luster M, Chae K, Chaudhary S, Clark G, Lawson L . A radioimmunoassay for chlorinated dibenzo-p-dioxins. Toxicol Appl Pharmacol. 1979; 50(1):137-46. DOI: 10.1016/0041-008x(79)90501-5. View

17.

Kumagai S, Koda S, Miyakita T, Ueno M . Polychlorinated dibenzo-p-dioxin and dibenzofuran concentrations in serum samples of workers at intermittently burning municipal waste incinerators in Japan. Occup Environ Med. 2002; 59(6):362-8. PMC: 1740303. DOI: 10.1136/oem.59.6.362. View

18.

Harrison R, Carlson R . An immunoassay for TEQ screening of dioxin/furan samples: current status of assay and applications development. Chemosphere. 1997; 34(5-7):915-28. DOI: 10.1016/s0045-6535(97)00395-0. View

19.

Zhao B, DeGroot D, Hayashi A, He G, Denison M . CH223191 is a ligand-selective antagonist of the Ah (Dioxin) receptor. Toxicol Sci. 2010; 117(2):393-403. PMC: 2940411. DOI: 10.1093/toxsci/kfq217. View

20.

Shelver W, Larsen G, Huwe J . Use of an immunoaffinity column for tetrachlorodibenzo-p-dioxin serum sample cleanup. J Chromatogr B Biomed Sci Appl. 1998; 705(2):261-8. DOI: 10.1016/s0378-4347(97)00540-9. View