Phospholipid Scramblase-1-induced Lipid Reorganization Regulates Compensatory Endocytosis in Neuroendocrine Cells

Overview

Journal J Neurosci

Specialty Neurology

Date 2013 Feb 22

PMID 23426682

Citations 27

Authors

Stephane Ory

Mara Ceridono

Fanny Momboisse

Sebastien Houy

Sylvette Chasserot-Golaz

Dimitri Heintz

Valerie Calco

Anne-Marie Haeberle

Flor A Espinoza

Peter J Sims

Yannick Bailly

Marie-France Bader

Stephane Gasman

Affiliations

Soon will be listed here.

Abstract

Calcium-regulated exocytosis in neuroendocrine cells and neurons is accompanied by the redistribution of phosphatidylserine (PS) to the extracellular space, leading to a disruption of plasma membrane asymmetry. How and why outward translocation of PS occurs during secretion are currently unknown. Immunogold labeling on plasma membrane sheets coupled with hierarchical clustering analysis demonstrate that PS translocation occurs at the vicinity of the secretory granule fusion sites. We found that altering the function of the phospholipid scramblase-1 (PLSCR-1) by expressing a PLSCR-1 calcium-insensitive mutant or by using chromaffin cells from PLSCR-1⁻/⁻ mice prevents outward translocation of PS in cells stimulated for exocytosis. Remarkably, whereas transmitter release was not affected, secretory granule membrane recapture after exocytosis was impaired, indicating that PLSCR-1 is required for compensatory endocytosis but not for exocytosis. Our results provide the first evidence for a role of specific lipid reorganization and calcium-dependent PLSCR-1 activity in neuroendocrine compensatory endocytosis.

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