Detection of Helicobacter Pylori in Gastric Aspirates Using a Monoclonal Antibody-Based Test

Overview

Journal Gut Liver

Specialty Gastroenterology

Date 2013 Feb 21

PMID 23423538

Citations 3

Authors

Ho Dong Kim

Do Hyun Kim

Hyeuk Park

Woo Jong Kim

Yong Soo Ahn

Young Jik Lee

Sun Mi Park

Eun Seon Seo

Chul Park

Yang Ho Kim

Hyung Rag Kim

Young Eun Joo

Young Do Jung

Affiliations

Soon will be listed here.

Abstract

Background/aims: The objective of this study was to evaluate a monoclonal antibody-based test to detect Helicobacter pylori-specific antigen in gastric aspirates from humans.

Methods: Sixty-one volunteers were enrolled in the study. All of the subjects underwent a (13)C-urea breath test (UBT) before esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia and used for polymerase chain reaction (PCR), culture, and monoclonal antibody-based detection of H. pylori. Multiple biopsies of the gastric antrum and body were obtained for a rapid urease test (RUT) and histological evaluation.

Results: Thirty-six subjects were H. pylori-positive and 25 were H. pylori-negative according to the UBT results. Compared with the H. pylori-negative subjects, H. pylori-positive subjects had a higher pH (4.77±1.77 vs 3.49±1.30, p<0.05) and ammonia level (1,130.9±767.4 vs 184.2±126.3, p<0.0001). The sensitivities and specificities of the PCR test, RUT, culture test, and monoclonal antibody-based test were 100% and 72%, 89% and 100%, 47% and 100%, and 78% and 100%, respectively.

Conclusions: The monoclonal antibody-based test for diagnosing H. pylori infection in gastric aspirates has increased sensitivity compared with the culture test and specificity as high as that of the RUT. The test may be useful as an additive test for examining gastric aspirates.

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